Molecular Breeding

, Volume 21, Issue 4, pp 511–532 | Cite as

Development of a genetic linkage map and identification of homologous linkage groups in sweetpotato using multiple-dose AFLP markers

  • Jim C. Cervantes-Flores
  • G. Craig Yencho
  • Albert Kriegner
  • Kenneth V. Pecota
  • Maria A. Faulk
  • Robert O. M. Mwanga
  • Bryon R. Sosinski
Article

Abstract

Sweetpotato genomic research is minimal compared to most other major crops despite its worldwide importance as a food crop. The development of a genetic linkage map in sweetpotato will provide valuable information about the genomic organization of this important species that can be used by breeders to accelerate the introgression of desired traits into breeding lines. We developed a mapping population consisting of 240 individuals of a cross between ‘Tanzania’, a cream-fleshed African landrace, and ‘Beauregard’, an orange-fleshed US sweetpotato cultivar. The genetic linkage map of this population was constructed using Amplified Fragment Length Polymorphism (AFLP) markers. A total of 1944 (‘Tanzania’) and 1751 (‘Beauregard’) AFLP markers, of which 1511 and 1303 were single-dose markers respectively, were scored. Framework maps consisting of 86 and 90 linkage groups for ‘Tanzania’ and ‘Beauregard’ respectively, were developed using a combination of JoinMap 3.0 and MAPMAKER/EXP 3.0. A total of 947 single-dose markers were placed in the final framework linkage map for ‘Tanzania’. The linkage map size was estimated as 5792 cM, with an average distance between markers of 4.5 cM. A total of 726 single-dose markers were placed in the final framework map for ‘Beauregard’. The linkage map length was estimated as 5276 cM, with an average distance between markers of 4.8 cM. Duplex and triple-dose markers were used to identify the corresponding homologous groups in the maps. Our research supports the hypothesis that sweetpotato is an autopolyploid. Distorted segregation in some markers of different dosages in this study suggests that some preferential pairing occurs in sweetpotato. However, strict allopolyploid inheritance in sweetpotato can be ruled out due to the observed segregation ratios of the markers, and the proportion of simplex to multiple-dose markers.

Keywords

Ipomoea batatas Molecular marker Molecular mapping Polyploid mapping Autopolyploid 

Notes

Acknowledgments

The research reported in this publication was supported by funding provided by the McKnight Foundation Collaborative Crops Research Project, the North Carolina SweetPotato Commission, Inc., the Consortium for Plant Biotechnology Research, and the GoldenLEAF Foundation. We thank Cindy Pierce, Jennifer Swift, Amanda Kroll, Megan Ulmer and Christina Rowe for their assistance in the greenhouse and laboratory.

Supplementary material

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11032_2007_9150_MOESM2_ESM.pdf (111 kb)
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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Jim C. Cervantes-Flores
    • 1
  • G. Craig Yencho
    • 1
  • Albert Kriegner
    • 2
  • Kenneth V. Pecota
    • 1
  • Maria A. Faulk
    • 1
  • Robert O. M. Mwanga
    • 3
  • Bryon R. Sosinski
    • 1
  1. 1.Department of Horticultural ScienceNorth Carolina State UniversityRaleighUSA
  2. 2.Austrian Research Centers, Seibersdorf G.m.b.H. Geschaftsfeld BioresourcesSeibersdorfAustria
  3. 3.National Crops Resources Research InstituteKampalaUganda

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