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Molecular Breeding

, Volume 20, Issue 4, pp 323–330 | Cite as

Molecular marker development and linkage analysis in three low phytic acid barley (Hordeum vulgare) mutant lines

  • Vicky Roslinsky
  • Peter E. EcksteinEmail author
  • Victor Raboy
  • Brian G. Rossnagel
  • Graham J. Scoles
Article

Abstract

Phytate is the primary form of phosphorus found in mature cereal grain. This form of phosphorus is not available to monogastric animals due to a lack of the enzyme phytase in their digestive tract. Several barley low phytic acid (lpa) mutants have been identified that contain substantial decreases in seed phytate accompanied by concomitant increases in inorganic phosphorus. Seed homozygous for low phytic acid 1-1 (lpa1-1) or low phytic acid 2-1 (lpa2-1) has a 50% and 70% decrease in seed phytate respectively. These mutations were previously mapped to chromosomes 2HL and 7HL respectively. The RFLP marker ABC153 located in the same region of 2H was converted to a sequence-characterized-amplified-region (SCAR) marker. Segregation analysis of the CDC McGwire × Lp422 doubled haploid population confirmed linkage between the SCAR marker and the lpa1-1 locus with 15% recombination. A third low phytic acid mutant, M635, has a 75% decrease in phytate. This mutation was located to chromosome 1HL by linkage with an inter-simple sequence repeat (ISSR) based marker (LP75) identified through bulked-segregant analysis, and has been designated lpa3-1. Based on analysis of recombination between marker LP75 and low phytic acid in an additional mutant line M955 (95% phytate decrease), lpa3-1 and the mutation in M955 are in the same region on chromosome 1HL, and may be allelic.

Keywords

Barley mutant Hordeum Low phytic acid Phosphorus Molecular marker 

Notes

Acknowledgement

We would like to thank Dr. Gene Arganosa for seed phytate analysis. This work was supported financially by the Saskatchewan Agriculture Development Fund and the Western Grains Research Foundation.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Vicky Roslinsky
    • 1
  • Peter E. Eckstein
    • 2
    Email author
  • Victor Raboy
    • 3
  • Brian G. Rossnagel
    • 2
  • Graham J. Scoles
    • 2
  1. 1.Saskatoon Research CentreAgriculture and Agri-Food CanadaSaskatoonCanada
  2. 2.Department of Plant Sciences/Crop Development Centre (CDC)University of SaskatchewanSaskatoonCanada
  3. 3.National Small Grains Germplasm Research FacilityUSDA-ARSAberdeenUSA

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