Molecular marker development and linkage analysis in three low phytic acid barley (Hordeum vulgare) mutant lines
- 152 Downloads
Phytate is the primary form of phosphorus found in mature cereal grain. This form of phosphorus is not available to monogastric animals due to a lack of the enzyme phytase in their digestive tract. Several barley low phytic acid (lpa) mutants have been identified that contain substantial decreases in seed phytate accompanied by concomitant increases in inorganic phosphorus. Seed homozygous for low phytic acid 1-1 (lpa1-1) or low phytic acid 2-1 (lpa2-1) has a 50% and 70% decrease in seed phytate respectively. These mutations were previously mapped to chromosomes 2HL and 7HL respectively. The RFLP marker ABC153 located in the same region of 2H was converted to a sequence-characterized-amplified-region (SCAR) marker. Segregation analysis of the CDC McGwire × Lp422 doubled haploid population confirmed linkage between the SCAR marker and the lpa1-1 locus with 15% recombination. A third low phytic acid mutant, M635, has a 75% decrease in phytate. This mutation was located to chromosome 1HL by linkage with an inter-simple sequence repeat (ISSR) based marker (LP75) identified through bulked-segregant analysis, and has been designated lpa3-1. Based on analysis of recombination between marker LP75 and low phytic acid in an additional mutant line M955 (95% phytate decrease), lpa3-1 and the mutation in M955 are in the same region on chromosome 1HL, and may be allelic.
KeywordsBarley mutant Hordeum Low phytic acid Phosphorus Molecular marker
We would like to thank Dr. Gene Arganosa for seed phytate analysis. This work was supported financially by the Saskatchewan Agriculture Development Fund and the Western Grains Research Foundation.
- Kasha KJ, Kleinhofs A (1994) Mapping of the barley cross Harrington × TR306. Barley Genet Newslett 23:65–69Google Scholar
- Kleinhofs A, Kilian A, Saghai Maroof MA, Biyashev RM, Hayes P, Chen FQ, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger J, Knapp SJ, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R, Steffenson BJ (1993) A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712CrossRefGoogle Scholar
- Procunier JD, Jie X, Kasha KJ (1990) A rapid and reliable DNA extraction method for higher plants. Barley Genet Newslett 20:74–75Google Scholar
- Raboy V (1997) Accumulation and storage of phosphate minerals. In: Larkins BA, Vasil IK (eds) Cellular and Molecular Biology of Plant Seed Development. Kluwer Academic Publishers, Netherlands, pp 441–477Google Scholar
- Thacker PA, Rossnagel BR, Raboy V (2003) Phosphorus digestibility in low-phytate barley fed to finishing pigs. Can J Anim Sci 83:101–104Google Scholar