Advertisement

Molecular Breeding

, Volume 20, Issue 4, pp 295–308 | Cite as

The successful application of a marker-assisted wheat breeding strategy

  • Haydn Kuchel
  • Rebecca Fox
  • Jason Reinheimer
  • Lee Mosionek
  • Nicholas Willey
  • Harbans Bariana
  • Stephen Jefferies
Article

Abstract

A number of useful marker-trait associations have been reported for wheat. However the number of publications detailing the integrated and pragmatic use of molecular markers in wheat breeding is limited. A previous report by some of these authors showed how marker-assisted selection could increase the genetic gain and economic efficiency of a specific breeding strategy. Here, we present a practical validation of that study. The target of this breeding strategy was to produce wheat lines derived from an elite Australian cultivar ‘Stylet’, with superior dough properties and durable rust resistance donated from ‘Annuello’. Molecular markers were used to screen a BC1F1 population produced from a cross between the recurrent parent ‘Stylet’ and the donor parent ‘Annuello’ for the presence of rust resistance genes Lr34/Yr18 and Lr46/Yr29. Following this, marker-assisted selection was applied to haploid plants, prior to chromosome doubling with cochicine, for the rust resistance genes Lr24/Sr24, Lr34/Yr18, height reducing genes, and for the grain protein genes Glu-D1 and Glu-A3. In general, results from this study agreed with those of the simulation study. Genetic improvement for rust resistance was greatest when marker selection was applied on BC1F1 individuals. Introgression of both the Lr34/Yr18 and Lr46/Yr29 loci into the susceptible recurrent parent background resulted in substantial improvement in leaf rust and stripe rust resistance levels. Selection for favourable glutenin alleles significantly improved dough resistance and dough extensibility. Marker-assisted selection for improved grain yield, through the selection of recurrent parent genome using anonymous markers, only marginally improved grain yield at one of the five sites used for grain yield assessment. In summary, the integration of marker-assisted selection for specific target genes, particularly at the early stages of a breeding programme, is likely to substantially increase genetic improvement in wheat.

Keywords

Dough quality Glutenin Marker-assisted selection Plant breeding Rust resistance Triticum aestivum 

Abbreviations

DH

Doubled haploid

HMW

High molecular weight

LMW

Low molecular weight

MAS

Marker-assisted selection

Notes

Acknowledgements

The authors would like to acknowledge the staff at Australian Grain Technologies, the University of Adelaide and the University of Sydney for there assistance collecting field, end-use quality, molecular marker and rust resistance data. Gratitude is also extended to the Molecular Plant Breeding Cooperative Research Centre and the Grains Research and Development Corporation for their financial assistance. The advice and direction of Prof. P. Langridge is gratefully acknowledged.

References

  1. Ahmad M (2000) Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers. Theor Appl Genet 101:892–896CrossRefGoogle Scholar
  2. Bariana HS, McIntosh RA (1993) Cytogenetic studies in wheat XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36:476–482PubMedGoogle Scholar
  3. Bonnett DG, Rebetzke GJ, Spielmeyer W (2005) Strategies for efficient implementation of molecular markers in wheat breeding. Mol Breed 15:75–85CrossRefGoogle Scholar
  4. Charmet G, Robert N, Perretant MR, Gay G, Sourdille P, Groos C, Bernard S, Bernard M (1999) Marker-assisted recurrent selection for cumulating additive and interactive QTLs in recombinant inbred lines. Theor Appl Genet 99:1143–1148CrossRefGoogle Scholar
  5. Devos KM, Bryan GJ, Collins AJ, Stephenson P, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252CrossRefGoogle Scholar
  6. Eagles HA, Hollamby GJ, Gororo NN, Eastwood RF (2002) Estimation and utilisation of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs. Aust J Agric Res. 53:367–377CrossRefGoogle Scholar
  7. Eglinton J, Coventry S, Chalmers K (2006) Breeding outcomes from molecular genetics. In: Mercer CF (ed) Proceedings of the 13th Australasian Plant Breeding Conference, Christchurch, 2006Google Scholar
  8. Ellis MH, Speilmeyer W, Gale KR, Rebetzke GJ, Richards RA (2002) “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor Appl Genet 105:1038–1042PubMedCrossRefGoogle Scholar
  9. Fan Z, Robbins MD, Staub JE (2006) Population development by phenotypic selection with subsequent marker-assisted selection for line extraction in cucumber (Cucumis sativus L.). Theor Appl Genet 112:843–855PubMedCrossRefGoogle Scholar
  10. Frisch M, Bohn M, Melchinger AE (1999) Comparison of selection strategies for marker-assisted backcrossing of a gene. Crop Sci 39:1295–1301CrossRefGoogle Scholar
  11. Frisch M, Melchinger AE (2005) Selection theory for marker-assisted backcrossing. Genetics 170:909–917PubMedCrossRefGoogle Scholar
  12. Gilmour AF, Cullis BR, Verbyla A (1997) Accounting for natural and extraneous variation in the analysis of field experiments. J Agr Biol Envir St 2:269–293CrossRefGoogle Scholar
  13. Gupta RB, MacRitchie F, Shepherd KW (1989) The cumulative effect of allelic variation in LMW and HMW glutenin subunits on dough properties in the progeny of two bread wheats. Theor Appl Genet 77:57–64CrossRefGoogle Scholar
  14. Hospital F, Moreau L, Lacourdre F, Charcosset A, Gallais A (1997) More on the efficiency of marker-assisted selection. Theor Appl Genet 95:1181–1189CrossRefGoogle Scholar
  15. Howes NK, Woods SM, Townley-Smith TF (1998) Simulations and practical problems of applying multiple marker assisted selection and doubled haploids to wheat breeding programs. Euphytica 100:225–230CrossRefGoogle Scholar
  16. Jefferies SP, King BJ, Barr AR, Warner P, Logue SJ, Langridge P (2003) Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley. Plant Breeding 122:52–56CrossRefGoogle Scholar
  17. Knapp SJ (1998) Marker-assisted selection as a strategy for increasing the probability of selecting superior genotypes. Crop Sci 38:1164–1174CrossRefGoogle Scholar
  18. Koebner RMD, Summers W (2003) 21st century wheat breeding: plot selection of plate detection? Trends Biotechnol 21:59–63PubMedCrossRefGoogle Scholar
  19. Korzun V, Roder MS, Ganal MW, Worland AJ, Law CN (1998) Genetic analysis of the dwarfing gene (Rht8) in wheat. Part 1. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theor Appl Genet 96:1104–1109CrossRefGoogle Scholar
  20. Kuchel H, Ye G, Fox R, Jefferies SP (2005) Genetic and economic analysis of a targeted marker-assisted wheat breeding strategy. Mol Breed 16:67–78CrossRefGoogle Scholar
  21. Mago R, Bariana HS, Dundas IS, Spielmeyer W, Lawrence GJ, Pryor AJ, Ellis JG (2005) Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theor Appl Genet 111:496–504PubMedCrossRefGoogle Scholar
  22. Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J Sci Food Agric 40:51–65CrossRefGoogle Scholar
  23. Payne RW, Baird DB, Cherry M, Gilmour AR, Harding SA, Kane AK, Lane PW, Murray DA, Soutar DM, Thompson R, Todd AD, Tunnicliffe Wilson G, Webster R, Welham SJ (2002) GenStat Rlease 6.1 Reference Manual. VSN International, Oxford, UKGoogle Scholar
  24. Ribaut JM, Jiang C, Hoisington D (2002) Simulation experiments on efficiencies of gene introgression by backcrossing. Crop Sci 42:557–565CrossRefGoogle Scholar
  25. Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  26. Roder MS, Plaschke J, Konig U, Borner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333PubMedCrossRefGoogle Scholar
  27. Rosewarne GM, Singh RP, Huerto-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508PubMedCrossRefGoogle Scholar
  28. Stam P, Zeven AC (1981) The theoretical proportion of the donor genome in near-isogenic lines of self-fertilizers bred by backcrossing. Euphytica 30:227–238CrossRefGoogle Scholar
  29. Somers DJ, Isaac P (2004) SSRs from the wheat microsatellite consortium. http://wheat.pw.usda.gov/ggpages/SSR/WMC/. Cited 20 Nov 2006Google Scholar
  30. Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890CrossRefPubMedGoogle Scholar
  31. Visscher PM (1999) Speed congenics: accelerated genome recovery using genetic markers. Genet Res 74:81–85PubMedCrossRefGoogle Scholar
  32. Yousef GG, Juvik JA (2001) Comparison of phenotypic and marker-assisted selection for quantitative traits in sweet corn. Crop Sci 41:645–655CrossRefGoogle Scholar
  33. Yu K, Park SJ, Poysa V (2000) Marker-assisted selection of common beans for resistance to common bacterial blight: efficacy and economics. Plant Breed 119:411–415CrossRefGoogle Scholar
  34. Zhou W-C, Kolb FL, Bai G-H, Dolmier LL, Boze LK, Smith NJ (2003) Validation of a major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat. Plant Breed 122:40–46CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Haydn Kuchel
    • 1
    • 2
    • 3
  • Rebecca Fox
    • 2
    • 3
  • Jason Reinheimer
    • 1
    • 2
  • Lee Mosionek
    • 1
  • Nicholas Willey
    • 4
  • Harbans Bariana
    • 4
  • Stephen Jefferies
    • 1
    • 2
    • 3
  1. 1.Australian Grain Technologies, Plant Breeding UnitRoseworthyAustralia
  2. 2.School of Agriculture, Food and WineUniversity of AdelaideGlen OsmondAustralia
  3. 3.Molecular Plant Breeding Cooperative Research CentreUniversity of AdelaideGlen OsmondAustralia
  4. 4.The University of Sydney Plant Breeding InstituteCamdenAustralia

Personalised recommendations