Co-expression of the Suaeda salsa SsNHX1 and Arabidopsis AVP1 confer greater salt tolerance to transgenic rice than the single SsNHX1
- 442 Downloads
Transgenic rice plants co-expressing the Suaeda salsa SsNHX1 (vacuolar membrane Na+/H+ antiporter) and Arabidopsis AVP1 (vacuolar H+-PPase) showed enhanced salt tolerance during 3 d of 300 mM NaCl treatment under outdoor growth conditions. These transgenic rice seedlings also grew better on MS medium containing 150 mM NaCl compared to SsNHX1-transformed lines and non-transformed controls. Measurements on isolated vacuolar membrane vesicles derived from the salt stressed SsNHX1+AVP1-transgenic plants demonstrated that the vesicles had increased V-PPase hydrolytic activity in comparison with the Ss-transgenics and non-transgenics. Moreover the V-PPase activity was closely related to the development period of the SA-transgenic seedlings and markedly higher in 3-week-old seedlings than in 5-week-old seedlings. Statistic analysis indicated that the SA-transgenic rice plants contained relatively more ions with higher K+/Na+ ratio in their shoots compared to the SsNHX1-transformed lines upon salt treatment. Furthermore, these SA-transformants also exhibited relatively higher level of photosynthesis and root proton exportation capacity whereas reduced H2O2 generation in the same plants. In general, these results supported the hypothesis that simultaneous expression of the SsNHX1 and AVP1 conferred greater performance to the transgenic plants than that of the single SsNHX1.
KeywordsAVP1 co-expression salt tolerance SsNHX1 Suaeda salsa transgenic rice
Unable to display preview. Download preview PDF.
- Barklaz BJ, Zingarelli L, Blumwald E, Smith JAC (1995) Tonoplast Na+/H+ antiport activity and its energization by the vacuolar H+-ATPase in the halophytic plant Mesembryanthemum crystallinum. Plant Physiol 109:549–556Google Scholar
- Fukuda A, Nakamura A, Tagiri A, Tanaka H, Miyao A, Hirochika H, Tanaka Y (2004) Function, intracellular localization and the importance in salt tolerance of a vacuolar Na+/H+ antiporter from rice. Plant Cell Physiol 45:149–159Google Scholar
- Liu QQ, Zang JL, Wang ZY, Hong MM, Gu MH (1998) A highly efficient transformation system mediated by Agrobacterium tumefaciens in Rice (Oryza sativa L.). Acta Phytophysiol Sin 24(3):259–271Google Scholar
- Lin ZF, Li SS, Lin GZ (1988) The accumulation of hydrogen peroxide in senescencing leaves and chloroplasts in relation to lipid peroxidation. Acta Phytophysiol Sin 14(1):16–22Google Scholar
- Maathuis FJ, Filatov V, Herzyk P, Krijger GC, Axelsen KB, Chen S, Green BJ, Li Y, Madagan KL, Sanchez-Fernandez R, Forde BG, Palmgren MG, Rea PA, Williams LE, Sanders D, Amtmann A (2003) Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress. Plant J 35(6):675–692PubMedCrossRefGoogle Scholar
- Maeshima M (1991) H(+)-translocating inorganic pyrophosphatase of plant vacuoles. Inhibition by Ca2+, stabilization by Mg2+ and immunological comparison with other inorganic pyrophosphatases. Eur J Biochem 196(1):11–17Google Scholar
- Papageorgiou GC, Alygizaki-Zorba A, Ladas N, Murata N (1998) A method to probe the cytoplasmic osmolality and osmotic water and solute fluxes across the cell membrane of cyanobacteria with Chl a fluorescence: experiments with Synechococcus sp. PCC 7942. Physiol Plant 103:215–224CrossRefGoogle Scholar