Molecular Breeding

, Volume 17, Issue 4, pp 341–353 | Cite as

Co-expression of the Suaeda salsa SsNHX1 and Arabidopsis AVP1 confer greater salt tolerance to transgenic rice than the single SsNHX1

  • Feng-Yun Zhao
  • Xue-Jie Zhang
  • Ping-Hua Li
  • Yan-Xiu Zhao
  • Hui Zhang
Article

Abstract

Transgenic rice plants co-expressing the Suaeda salsa SsNHX1 (vacuolar membrane Na+/H+ antiporter) and Arabidopsis AVP1 (vacuolar H+-PPase) showed enhanced salt tolerance during 3 d of 300 mM NaCl treatment under outdoor growth conditions. These transgenic rice seedlings also grew better on MS medium containing 150 mM NaCl compared to SsNHX1-transformed lines and non-transformed controls. Measurements on isolated vacuolar membrane vesicles derived from the salt stressed SsNHX1+AVP1-transgenic plants demonstrated that the vesicles had increased V-PPase hydrolytic activity in comparison with the Ss-transgenics and non-transgenics. Moreover the V-PPase activity was closely related to the development period of the SA-transgenic seedlings and markedly higher in 3-week-old seedlings than in 5-week-old seedlings. Statistic analysis indicated that the SA-transgenic rice plants contained relatively more ions with higher K+/Na+ ratio in their shoots compared to the SsNHX1-transformed lines upon salt treatment. Furthermore, these SA-transformants also exhibited relatively higher level of photosynthesis and root proton exportation capacity whereas reduced H2O2 generation in the same plants. In general, these results supported the hypothesis that simultaneous expression of the SsNHX1 and AVP1 conferred greater performance to the transgenic plants than that of the single SsNHX1.

Keywords

AVP1 co-expression salt tolerance SsNHX1 Suaeda salsa transgenic rice 

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Copyright information

© Springer Science + Business Media B.V. 2006

Authors and Affiliations

  • Feng-Yun Zhao
    • 1
    • 2
  • Xue-Jie Zhang
    • 1
  • Ping-Hua Li
    • 1
  • Yan-Xiu Zhao
    • 1
  • Hui Zhang
    • 1
  1. 1.Key Laboratory of Plant Stress, Life Science CollegeShandong Normal UniversityJinanP. R. China
  2. 2.Life Science CollegeShandong Science and Engineering UniversityZiboP. R. China

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