Molecular Breeding

, Volume 17, Issue 2, pp 173–184 | Cite as

Integration of Retrotransposons-Based Markers in a Linkage Map of Barley

  • Monica Rodriguez
  • Donal O’Sullivan
  • Paolo Donini
  • Roberto Papa
  • Elena Chiapparino
  • Fiona Leigh
  • Giovanna Attene
Article

Abstract

A deeper understanding of random markers is important if they are to be employed for a range of objectives. The sequence specific amplified polymorphism (S-SAP) technique is a powerful genetic analysis tool which exploits the high copy number of retrotransposon long terminal repeats (LTRs) in the plant genome. The distribution and inheritance of S-SAP bands in the barley genome was studied using the Steptoe × Morex (S × M) double haploid (DH) population. Six S-SAP primer combinations generated 98 polymorphic bands, and map positions were assigned to all but one band. Eight putative co-dominant loci were detected, representing 16 of the mapped markers. Thus at least 81 of the mapped S-SAP loci were dominant. The markers were distributed along all of the seven chromosomes and a tendency to cluster was observed. The distribution of S-SAP markers over the barley genome concurred with the knowledge of the high copy number of retrotransposons in plants. This experiment has demonstrated the potential for the S-SAP technique to be applied in a range of analyses such as genetic fingerprinting, marker assisted breeding, biodiversity assessment and phylogenetic analyses.

Keywords

Barley Molecular mapping Retrotransposons S-SAP (Sequence-Specific Amplified Polymorphism) markers 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Monica Rodriguez
    • 1
  • Donal O’Sullivan
    • 2
  • Paolo Donini
    • 2
  • Roberto Papa
    • 3
  • Elena Chiapparino
    • 2
  • Fiona Leigh
    • 2
  • Giovanna Attene
    • 1
  1. 1.Dipartimento di Scienze Agronomiche e Genetica Vegetale AgrariaUniversità degli Studi di SassraiSassariItaly
  2. 2.Molecular Research Group, NIABCambridgeUK
  3. 3.Dipartimento di Scienze degli AlimentiUniversità Politecnica delle MarcheVia Brecce BiancheItaly

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