Molecular Breeding

, Volume 17, Issue 2, pp 149–157 | Cite as

SSR Markers Closely Linked to the Pi-z Locus are Useful for Selection of Blast Resistance in a Broad Array of Rice Germplasm

  • Robert Fjellstrom
  • Anna M. McClung
  • A. Robert Shank


Pi-z is a disease resistance gene that has been effectively used to combat a broad-spectrum of races of the rice blast fungus Magnaporthe grisea. Although DNA markers have been reported for selection of the Pi2(t) and Pi-z resistance genes at the Pi-z locus, markers that are more tightly linked to the Pi-z locus would benefit rapid and effective cultivar development. Analysis of the publicly available genome sequence of Nipponbare near the Pi-z locus revealed numerous SSRs that could be converted into markers. Three SSRs on rice PAC AP005659 were found to be very tightly linked to the Pi-z locus, with one marker, AP5659-3, co-segregating with the Pi-z resistance reaction. The Pi-z factor conferring resistance to two races of blast was mapped to a 57 kb region on the physical map of Nipponbare in a location where the Pi2(t) gene was physically mapped. Two SSR marker haplotypes were unique for cultivars carrying the Pi-z gene, which indicates these markers are useful for selection of resistance genes at the Pi-z locus in rice germplasm.

Key words

Magnaporthe grisea Marker assisted selection Microsatellite Oryza sativa 



simple sequence repeat


bacterial/P1-derived artificial chromosome


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Copyright information

© Springer 2006

Authors and Affiliations

  • Robert Fjellstrom
    • 1
  • Anna M. McClung
    • 1
  • A. Robert Shank
    • 1
  1. 1.United States Department of AgricultureAgricultural Research Service, Rice Research Unit BeaumontUSA

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