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Molecular Breeding

, Volume 17, Issue 2, pp 149–157 | Cite as

SSR Markers Closely Linked to the Pi-z Locus are Useful for Selection of Blast Resistance in a Broad Array of Rice Germplasm

  • Robert Fjellstrom
  • Anna M. McClung
  • A. Robert Shank
Article

Abstract

Pi-z is a disease resistance gene that has been effectively used to combat a broad-spectrum of races of the rice blast fungus Magnaporthe grisea. Although DNA markers have been reported for selection of the Pi2(t) and Pi-z resistance genes at the Pi-z locus, markers that are more tightly linked to the Pi-z locus would benefit rapid and effective cultivar development. Analysis of the publicly available genome sequence of Nipponbare near the Pi-z locus revealed numerous SSRs that could be converted into markers. Three SSRs on rice PAC AP005659 were found to be very tightly linked to the Pi-z locus, with one marker, AP5659-3, co-segregating with the Pi-z resistance reaction. The Pi-z factor conferring resistance to two races of blast was mapped to a 57 kb region on the physical map of Nipponbare in a location where the Pi2(t) gene was physically mapped. Two SSR marker haplotypes were unique for cultivars carrying the Pi-z gene, which indicates these markers are useful for selection of resistance genes at the Pi-z locus in rice germplasm.

Key words

Magnaporthe grisea Marker assisted selection Microsatellite Oryza sativa 

AbbreviationGroup

SSR

simple sequence repeat

BAC/PAC

bacterial/P1-derived artificial chromosome

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References

  1. Conaway-Bormans, C.A., Marchetti, M.A., Johnson, C.W., McClung, A.M., Park, W.D. 2003Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selectionTheor. Appl. Genet.10710141020CrossRefPubMedGoogle Scholar
  2. Hayashi, K., Hashimoto, N., Daigen, M., Ashikawa, I. 2004Development of PCR-based SNP markers for rice blast resistance genes at the Piz locusTheor. Appl. Genet.10812121220PubMedGoogle Scholar
  3. Hittalmani, S., Foolad, M.R., Mew, T., Rodriguez, R.L., Huang, N. 1995Development of a PCR-based marker to identify rice blast resistance genePi-2(t)in a segregating populationTheor. Appl. Genet.91914CrossRefGoogle Scholar
  4. Inukai, T., Nelson, R.J., Ziegler, R.S., Sarkarung, S., Mackill, D.J., Bonman, J.M., Takamure, I., Kinoshita, T. 1994Allelism of blast resistance genes in near-isogenic lines of ricePhytopathology8412781283Google Scholar
  5. Kiyosawa, S. 1967The inheritance of resistance of the Zenith type varieties of rice to the blast fungusJap. J. Breed.1799107Google Scholar
  6. Liu, G., Lu, G., Zeng, L., Wang, G.-L. 2002Two broad-spectrum blast resistance genes, Pi9(t)Pi2(t)are physically linked on rice chromosome 6Mol. Genet. Genom.267472480Google Scholar
  7. Manly, K.F., Cudmore, R.H.,Jr., Meer, J.M. 2001Map Manager QTX, cross-platform software for genetic mappingMamm. Genome12930932CrossRefPubMedGoogle Scholar
  8. Marchetti, M.A., Lai, X., Bollich, C.N. 1987Inheritance of resistance to Pyricularia oryzae in rice cultivars grown in the United StatesPhytopathology77799804Google Scholar
  9. McClung, A.M. 2002Techniques for development of new cultivarsSmith, C.W.Dilday, R.H. eds. Rice: Origin, History, Technology and ProductionJohn Wiley & SonsHoboken, NJ177202Google Scholar
  10. Rozen, S., Skaletsky, H.J. 2000Primer3 on the WWW for general users and for biologist programmersKrawetz, S.Misener, S. eds. Bioinformatics Methods and Protocols: Methods in Molecular BiologyHumana PressTotowaNJ365386Google Scholar
  11. Temnykh, S., DeClerck, G., Lukashova, A., Lipovich, L., Cartinhour, S., McCouch, S. 2001Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potentialGenome Res.1114411452CrossRefPubMedGoogle Scholar
  12. Yu, Z.H., Mackill, D.J., Bonman, J.M., Tanksley, S.D. 1991Tagging genes for blast resistance in rice via linkage to RFLP markersTheor. Appl. Genet.81471476CrossRefGoogle Scholar
  13. Ware, D., Jaiswal, P., Ni, J., Pan, X., Chang, K., Clark, K., Teytelman, L., Schmidt, S., Zhao, W., Cartinhour, S., McCouch, S., Stein, L. 2002Gramene: a resource for comparative grass genomicsNucleic Acids Res.30103105CrossRefPubMedGoogle Scholar
  14. Zhou, B., Qu, S., Liu, G., Dolan, M., Sakai, H., Bellizzi, M., Han, B., Wang, G.-L. 2004Functional and evolutional analysis of the Pi2 blast resistance gene clusterProc. Plant & Animal Genome Conf. XIISan DiegoCA399Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Robert Fjellstrom
    • 1
  • Anna M. McClung
    • 1
  • A. Robert Shank
    • 1
  1. 1.United States Department of AgricultureAgricultural Research Service, Rice Research Unit BeaumontUSA

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