Molecular Breeding

, Volume 17, Issue 2, pp 91–99 | Cite as

Heterologous Expression of Two Gentian Cytochrome P450 Genes can Modulate the Intensity of Flower Pigmentation in Transgenic Tobacco Plants

  • Takashi Nakatsuka
  • Masahiro Nishihara
  • Keiichiro Mishiba
  • Saburo Yamamura
Article

Abstract

Two different heterologous expression systems, microsomal fractions of Saccharomyces cerevisiae and transgenic tobacco plants, were used to investigate the enzymatic activities of flavonoid 3′-hydroxylase (GtF3′H) and flavone synthase II (GtFSII) homologues isolated from gentian petals. Recombinant GtF3′H expressed in yeast showed hydroxylation activities in the 3′ position with several flavonoid substrates, while recombinant GtFSII was able to produce flavone from flavanone. GtF3′ H-expressing transgenic tobacco plants showed a slight increase in anthocyanin content and flower color intensity, and conversion of the flavonol quercetin from kaempferol. On the other hand, GtFSII-expressing plants showed a remarkable reduction in anthocyanin content and flower color intensity, and additional accumulation of flavone, especially luteolin derivatives. We demonstrated that two cytochrome P450s from gentian petals have F3′H and FSII enzymatic activities both in vitro and in vivo, and might therefore be useful in modification of flower color using genetic engineering.

Key words

Cytochrome P450 Flavone synthase II Flavonoid 3′-hydroxylase Flavonoid biosynthesis Gentiana triflora Nicotiana tabacum 

Abbreviations

DHK

dihydrokaempferol

DHM

dihydromyricetin

DHQ

dihydroquercetin

F3′H

flavonoid 3′-hydroxylase

FSII

flavone synthase II

HPLC

high performance liquid chromatography

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Copyright information

© Springer 2006

Authors and Affiliations

  • Takashi Nakatsuka
    • 1
  • Masahiro Nishihara
    • 1
  • Keiichiro Mishiba
    • 1
    • 2
  • Saburo Yamamura
    • 1
  1. 1.Iwate Biotechnology Research CenterKitakamiJapan
  2. 2.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversitySakaiJapan

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