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Molecular Breeding

, Volume 16, Issue 4, pp 285–293 | Cite as

Development of Biosafe Genetically Modified Solanum tuberosum (Potato) Cultivars for Growth within a Centre of Origin of the Crop

  • Jayne Green
  • Mandy T. Fearnehough
  • Howard J. Atkinson
Article

Abstract

Male sterile cv Desiree has been generated for use with other transgenic traits where hybridisation of a potato crop with wild relatives might occur. A ribosomal inactivating protein from maize (b32RIP) was expressed under the control of a tapetal specific promoter (TA29). Its known low activity against plant ribosomes was not correlated with either a lower incidence or less complete male sterility than occurred for similar lines expressing an RNAse (barnase) from Bacillus amyloliquefaciens. The b32RIP lines had a lower incidence of abnormal vegetative growth than barnase lines. Pollen grains of eight b32RIP lines and two barnase lines rarely showed uptake of the fluorophore fluorescein diacetate unlike the wild type Desiree. Pollen tubes formed very infrequently for sterile lines in conditions where this occurred for >80% of pollen of an untransformed control. Hand crossing with pollen from wild type but not the male sterile lines achieved fertilisation. Histological section of anthers for one b32RIP line confirmed its pollen grains had not matured in contrast to control plants. This is the first report of b32RIP having activity in planta against plant cells.

Keywords

Barnase Male sterility Nematode resistance Ribosomal inactivating protein Tapetal specific promoter 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Jayne Green
    • 1
  • Mandy T. Fearnehough
    • 1
  • Howard J. Atkinson
    • 1
  1. 1.Centre for Plant SciencesUniversity of LeedsLeedsUK

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