Molecular Breeding

, Volume 15, Issue 2, pp 145–156 | Cite as

Mapping of quantitative trait loci controlling adaptive traits in coastal Douglas-fir. IV. Cold-hardiness QTL verification and candidate gene mapping

  • Nicholas C. WheelerEmail author
  • Kathleen D. Jermstad
  • Konstantin Krutovsky
  • Sally N. Aitken
  • Glenn T. Howe
  • Jodie Krakowski
  • David B. Neale


Quantitative trait locus (QTL) analyses are used by geneticists to characterize the genetic architecture of quantitative traits, provide a foundation for marker-aided-selection (MAS), and provide a framework for positional selection of candidate genes. The most useful QTL for breeding applications are those that have been verified in time, space, and/or genetic background. In this study, spring cold-hardiness of Douglas-fir foliar tissues was evaluated in two clonally replicated (n=170 and 383 clones) full-sib cohorts derived from the same parental cross in two different years (made 5 years apart). The cohorts were established in widely separated forest test sites and tissues were artificially freeze tested using different cold injury assessment methods. Four of six unique QTL detected for spring cold-hardiness in needles of Cohort 1 were tentatively verified in the second cohort. Four additional QTL were detected in Cohort 2, two on linkage groups (LGs) not previously represented in the smaller cohort. In total, 10 unique QTL were identified across both cohorts. Seventeen of twenty-nine putative cold-hardiness candidate genes (Douglas-fir ESTs) placed on the Douglas-fir linkage map locate within the 95% confidence intervals of spring needle cold-hardiness QTL from the two cohorts and thus represent priority targets for initiating association mapping in Douglas-fir.


Association Genetics Candidate Gene Cold-hardiness Douglas-fir MAS QTL Verification 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Nicholas C. Wheeler
    • 1
    Email author
  • Kathleen D. Jermstad
    • 2
  • Konstantin Krutovsky
    • 2
  • Sally N. Aitken
    • 3
  • Glenn T. Howe
    • 4
  • Jodie Krakowski
    • 3
  • David B. Neale
    • 2
    • 5
  1. 1.Molecular Tree Breeding Services, LLCCentraliaUSA
  2. 2.Institute of Forest Genetics, Pacific Southwest Research StationUSDA Forest ServiceDavisUSA
  3. 3.Department of Forest Sciences, Faculty of ForestryUniversity of British ColumbiaVancouverCanada
  4. 4.Department of Forest ScienceOregon State UniversityCorvallisUSA
  5. 5.Department of Environmental HorticultureUniversity of CaliforniaDavisUSA

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