, Volume 74, Issue 3, pp 265–269 | Cite as

A Study of the Mechanism of Acetate Assimilation in Purple Nonsulfur Bacteria Lacking the Glyoxylate Shunt: Acetate Assimilation in Rhodobacter sphaeroides

  • L. V. Filatova
  • I. A. Berg
  • E. N. Krasil’nikova
  • A. A. Tsygankov
  • T. V. Laurinavichene
  • R.N. IvanobskyEmail author
Experimental Articles


The mechanism of acetate assimilation in the purple nonsulfur bacterium Rhodobacter sphaeroides, which lacks the glyoxylate shunt, has been studied. It has been found that the growth of this bacterium in batch and continuous cultures and the assimilation of acetate in cell suspensions are not stimulated by bicarbonate. The consumption of acetate is accompanied by the excretion of glyoxylate and pyruvate into the medium, stimulated by glyoxylate and pyruvate, and inhibited by citramalate. The respiration of cells in the presence of acetate is stimulated by glyoxylate, pyruvate, citramalate, and mesaconate. These data suggest that the citramalate cycle may function in Rba. sphaeroides in the form of an anaplerotic pathway instead of the glyoxylate shunt. At the same time, the low ratio of fixation rates for bicarbonate and acetate exhibited by the Rba. sphaeroides cells (approximately 0.1), as well as the absence of the stimulatory effect of acetate on the fixation of bicarbonate in the presence of the Calvin cycle inhibitor iodoacetate, suggests that pyruvate synthase is not involved in acetate assimilation in the bacterium Rba. sphaeroides.

Key words

Rhodobacter sphaeroides citramalate pathway glyoxylate shunt acetate 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cronan, J.E. and LaPorte, D., Tricarboxylic Acid Cycle and Glyoxylate Bypass, Escherichia coli and Salmonella: Cellular and Molecular Biology, Neidhardt, F.C. et al., Eds., Washington: Am. Soc. Microbiol., 1996, pp. 206–216.Google Scholar
  2. 2.
    Albers, H. and Gottschalk, G., Acetate Metabolism in Rhodopseudomonas gelatinosa and Several Other Rhodospirillaceae, Arch. Microbiol., 1976, vol. 111, pp. 45–49.Google Scholar
  3. 3.
    Kornberg, H.L. and Lascelles, J., The Formation of Isocitratase by the Athiorhodaceae, J. Gen. Microbiol., 1960, vol. 23, pp. 511–517.Google Scholar
  4. 4.
    Dunstan, P.M., Anthony, C., and Drabble, W.T., Microbial Metabolism of C1 and C2 Compounds: The Involvement of Glycollate in the Metabolism of Ethanol and of Acetate by Pseudomonas AM1, Biochem. J., 1972, vol. 128, pp. 99–106.Google Scholar
  5. 5.
    Korotkova, N., Chistoserdova, L., Kuksa, V., and Lidstrom, M.E., Glyoxylate Regeneration Pathway in the Methylotroph Methylobacterium extorquens AM1, J. Bacteriol., 2002, vol. 184, pp. 1750–1758.Google Scholar
  6. 6.
    Berg, I.A., Krasil’nikova, E.N., and Ivanovskii, R.N., Investigation of the Dark Metabolism of Acetate in Photoheterotrophically Grown Cells of Rhodospirillum rubrum, Mikrobiologiya, 2000, vol. 69, pp. 13–18.Google Scholar
  7. 7.
    Ivanovskii, R.N., Krasil’nikova, E.N., and Berg, I.A., The Mechanism of Acetate Assimilation in the Purple Nonsulfur Bacterium Rhodospirillum rubrum Lacking Isocitrate Lyase, Mikrobiologiya, 1997, vol. 66, pp. 744–749.Google Scholar
  8. 8.
    Ivanovsky, R.N., Krasilnikova, E.N., and Berg, I.A., A Proposed Citramalate Cycle for Acetate Assimilation in the Purple Non-Sulfur Bacterium Rhodospirillum rubrum, FEMS Microbiol. Lett., 1997, vol. 153, pp. 399–404.Google Scholar
  9. 9.
    Berg, I.A., Filatova, L.V., and Ivanovsky, R.N., Inhibition of Acetate and Propionate Assimilation by Itaconate via Propionyl-CoA Carboxylase in the Isocitrate Lyase-Negative Purple Bacterium Rhodospirillum rubrum, FEMS Microbiol. Lett., 2002, vol. 216, pp. 49–54.Google Scholar
  10. 10.
    Bergey’s Manual of Systematic Bacteriology, 2nd ed., Boone, D.R. et al., Eds., New York: Springer, 2001, vol. 1.Google Scholar
  11. 11.
    Tsygankov, A.A., Laurinavichene, T.V., and Gogotov, I.N., Laboratory Scale Photobioreactor, Biotechnol. Technique, 1994, vol. 8, pp. 575–578.Google Scholar
  12. 12.
    Kornberg, H.L. and Gotto, A.M., The Metabolism of C2 Compounds in Microorganisms: 6. Synthesis of Cell Constituents from Glycollate by Pseudomonas sp., Biochem. J., 1961, vol. 78, pp. 69–82.Google Scholar
  13. 13.
    Dixon, G.H. and Kornberg, H.L., Assay Methods for Key Enzymes of the Glyoxylate Cycle, Biochem. J., 1959, vol. 72, pp. 195–200.Google Scholar
  14. 14.
    Giachetti, E., Pinzanti, G., and Vanni, P., A New Continuous Optical Assay for Isocitrate Lyase, Experientia, 1984, vol. 40, pp. 227–228.Google Scholar
  15. 15.
    Blasco, R., Cardenas, J., and Castillo, F., Acetate Metabolism in Purple Non-Sulfur Bacteria, FEMS Microbiol. Lett., 1989, vol. 58, pp. 129–132.Google Scholar
  16. 16.
    Eley, J.H., Knobloch, K., and Han, T.W., The Effect of Growth Conditions on Enzymes of the Citric Acid Cycle and the Glyoxylate Cycle in the Photosynthetic Bacterium Rhodopseudomonas palustris, Antonie van Leeuwenhoek, 1979, vol. 45, pp. 521–529.Google Scholar
  17. 17.
    Ferri, G., Comerio, G., Iadarola, P., Zapponi, M.C., and Speranza, M.L., Subunit Structure and Activity of Glyceraldehyde-3-Phosphate Dehydrogenase from Spinach Chloroplasts, Biochim. Biophys. Acta, 1978, vol. 522, pp. 19–31.Google Scholar
  18. 18.
    Kondratieva, E.N., Ivanovsky, R.N., and Krasilnikova, E.N., Light and Dark Metabolism in Purple Sulfur Bacteria, Soviet Science Review, Skulachev, V.P., Ed., New York: IPC, 1981, pp. 325–364.Google Scholar
  19. 19.
    Firsov, N.N. and Ivanovsky, R.N., Photometabolism of Acetate in Ectothiorhodospira shaposhnikovii, Mikrobiologiya, 1975, vol. 44, pp. 197–201.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • L. V. Filatova
    • 1
  • I. A. Berg
    • 1
  • E. N. Krasil’nikova
    • 1
  • A. A. Tsygankov
    • 2
  • T. V. Laurinavichene
    • 2
  • R.N. Ivanobsky
    • 1
    Email author
  1. 1.Department of Microbiology, Faculty of BiologyMoscow State UniversityVorob’evy gory, MoscowRussia
  2. 2.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow oblastRussia

Personalised recommendations