Measurement Techniques

, Volume 55, Issue 12, pp 1425–1433 | Cite as

The Method of Calibration Curves for Immunochromatographic Express Tests. Part 1. Immunochromatographic Express Tests with Colloidal Gold

  • S. S. Golubev
  • B. B. Dzantiev
  • A. V. Zherdev
  • Yu. V. Kiseleva
  • Ya. A. Korolenko
  • Yu. A. Kudeyarov
  • V. M. Malyuchenko
  • N. I. Smirnova
  • D. V. Sotnikov
Article
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The operating principle of immunochromatographic test systems is considered. It is noted that with the use of such tests an immunoassay is implemented as a sandwich method in the form of a direct dependence of the luminescence of test stripes on the concentration of the test substance or as a competitive method with an inverse dependence. An experimental procedure of obtaining such dependences, which is necessary for the construction of calibration curves, is set forth. Exponential expressions for arriving at an analytic description of the associated curves are proposed; moreover, the coefficients in these expressions are determined by the method of least squares. An analysis of the error of the method of calibration curves that may be used to obtain quantitative results of the analysis is presented.

Keywords

immunochromatographic express tests test and controls strips sandwich and competitive methods standard dilutions colloidal gold calibration curves 
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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • S. S. Golubev
    • 1
  • B. B. Dzantiev
    • 2
  • A. V. Zherdev
    • 2
  • Yu. V. Kiseleva
    • 1
  • Ya. A. Korolenko
    • 1
  • Yu. A. Kudeyarov
    • 1
  • V. M. Malyuchenko
    • 1
  • N. I. Smirnova
    • 2
  • D. V. Sotnikov
    • 2
  1. 1.All-Russia Research Institute of Metrological Service (VNIIMS)MoscowRussia
  2. 2.Bakh Institute of BiochemistryRussian Academy of SciencesMoscowRussia

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