Measurement Techniques

, Volume 55, Issue 6, pp 731–733 | Cite as

Use of nanoparticles for studying hemoglobin

  • G. V. Maksimov
  • N. A. Braze
  • A. I. Yusipovich
  • E. Yu. Parshina
  • O. V. Rodnenkov
  • A. B. Rubin
  • G. G. Levin
  • V. A. Bykov
Article
  • 73 Downloads

We present the advantages and features of comprehensive application of atomic force microscopy, laser interference microscopy, and Raman microscopy to study erythrocytes. We have determined the effectiveness of signal enhancement as a function of the size of silver nanoparticles in the Raman method. We consider the feasibility of using the developed techniques for clinical diagnosis.

Keywords

erythrocyte hemoglobin Raman scattering silver colloids 

Notes

This work was done with the financial support from the Ministry of Education and Science of the Russian Federation (State Contract No. 16.552.11.7049).

References

  1. 1.
    G. V. Maksimov et al., “Study of demyelination of nerve fiber using dynamic phase microscopy,” Byull. Eksperim. Biol. Meditsiny, 131, No. 5, 539–542 (2001).Google Scholar
  2. 2.
    V. P. Tychinsky et al., “A dynamic phase microscopy study of optical characteristics of individual chloroplasts,” Biochim. Biophys. Acta, 1665, 57 (2004).CrossRefGoogle Scholar
  3. 3.
    L. A. Erokhova et al., “Application of laser interference microscopy to study regular intracellular and membrane processes in neurons,” Byull. Eksperim. Biol. Meditsiny, 140, No. 8, 237 (2005).Google Scholar
  4. 4.
    N. A. Brazhe et al., “Unravelling cell processes: interference imaging interwoven with data-analysis,” J. Biolog. Phys., 32, 191 (2006).CrossRefGoogle Scholar
  5. 5.
    A. I. Yusipovich et al., “Features of investigation of an isolated neuron by laser interference microscopy,” Kvant. Elektron., 36, No. 9, 874–878 (2006).ADSCrossRefGoogle Scholar
  6. 6.
    G. G. Levin, F. V. Bulygin, and G. N. Vishnyakov, “Coherent oscillations in the state of protein molecules in living cells,” Tsitologiya, 47, No. 4, 348–356 (2005).Google Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • G. V. Maksimov
    • 1
  • N. A. Braze
    • 1
  • A. I. Yusipovich
    • 1
  • E. Yu. Parshina
    • 1
  • O. V. Rodnenkov
    • 2
  • A. B. Rubin
    • 1
  • G. G. Levin
    • 3
  • V. A. Bykov
    • 4
  1. 1.Lomonosov Moscow State UniversityMoscowRussia
  2. 2.Russian Cardiology Scientific and Production CenterMoscowRussia
  3. 3.All-Russia Research Institute of Optophysical Measurements (VNIIOFI)MoscowRussia
  4. 4.NT-MDT CompanyMoscowRussia

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