Evaluation of neuroglobin and cytoglobin expression in adult rats exposed to silver nanoparticles during prepubescence

  • Rodrigo Rodrigues da ConceiçãoEmail author
  • Janaina Sena de Souza
  • Kelen Carneiro de Oliveira
  • Renata Marino Romano
  • Rui Monteiro de Barros Maciel
  • Magnus Régios Dias-da-Silva
  • Marco Aurélio Romano
  • Maria Izabel Chiamolera
  • Gisele Giannocco
Original Article


Silver nanoparticles (AgNPs) are clusters of silver atoms with diameters that range from 1 to 100 nm. Due to the various shapes and large surface areas, AgNPs have been employed in the food and textile industries and medical fields. Therefore, because of the widespread use of these compounds, the aim of this study was to evaluate the effect of AgNP exposure on the gene and protein expression levels of Neuroglobin (Ngb) and Cytoglobin (Cygb), in the rat cortex, hippocampus and cerebellum. Post-natal day (PND) 21 male Wistar rats were randomly divided into three groups. One group received 15 μg/kg body weight of AgNP by gavage another group received 30 μg/kg and the control group that received saline, from PND23 to PND58. On PND102 the animals were euthanized and the cortex, hippocampus and cerebellum were isolated and evaluated for gene and protein expression levels of Nbg and Cygb. The results demonstrated that the 30 μg/kg AgNP group displayed increased gene and protein expression of Cygb in the cortex. In the Hippocampus, AgNP exposure did not modulate gene or protein expression levels of Ngb and Cygb. In cerebellum the Ngb gene and protein expression was increased with both doses of AgNP. AgNP exposure during prepubescence can modulate the gene and protein expression levels of Ngb and Cygb in adulthood. Furthermore, the observed modulation was specific to the cerebellum, and cortex, and was dose dependent.


Silver nanoparticle Neuroglobin Cytoglobin Brain 



We thank the Coordenação de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for fincancial support through the grant to MRDS (23038009864/2013-98) and the scholarships to RRC, JSS, and KCO. We would also like to thank the Sao Paulo Research Foundation (FAPESP) for supporting this work through the research grant to MIC (2013/26851-7) and the scholarships to JSS (FAPESP 18952-21-7).

Compliance with ethical standards

Conflict of interest

We wish to confirm that there are no conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rodrigo Rodrigues da Conceição
    • 1
    Email author
  • Janaina Sena de Souza
    • 1
  • Kelen Carneiro de Oliveira
    • 1
  • Renata Marino Romano
    • 2
  • Rui Monteiro de Barros Maciel
    • 1
  • Magnus Régios Dias-da-Silva
    • 1
  • Marco Aurélio Romano
    • 2
  • Maria Izabel Chiamolera
    • 1
  • Gisele Giannocco
    • 1
    • 3
  1. 1.Laboratório de Endocrinologia Molecular e Translacional, Departamento de Medicina, Disciplina de Endocrinologia ClínicaUniversidade Federal de São Paulo (UNIFESP)São PauloBrazil
  2. 2.Laboratory of Reproductive Toxicology, Department of PharmacyState University of Centro-OesteParanaBrazil
  3. 3.Departamento de Ciências BiológicasUniversidade Federal de São PauloDiademaBrazil

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