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Prior exercise protects against oxidative stress and motor deficit in a rat model of Parkinson’s disease

  • Leonam Oliver Durval OliveiraEmail author
  • Pedro Iuri Castro da Silva
  • Renato Pereira Rodrigues Filho
  • Raphaely Cristiny Sanches Progênio
  • Victor Douglas Pereira Silva de Oliveira
  • Renata Cunha Silva
  • Mariseth Carvalho de Andrade
  • Jofre Jacob da Silva Freitas
  • Katia Simone Kietzer
Original Article

Abstract

This study investigated if a prior long-term physical exercise protocol protects the substantia nigra and the striatum against oxidative stress and motor deficits in a Parkinson Disease model induced by 6-hydroxydopamine. Three animal treatment groups were included in the study: sham; 6-hydroxydopamine and 6-hydroxydopamine/exercise. Previously to the intrastriatal lesion by 6-hydroxydopamine, rats in the exercise groups performed a swimming program for 18 weeks. The rats were submitted to behavioral tests before and after intrastriatal 6-hydroxydopamine injection. The oxidative stress was analyzed by Thiobarbituric Acid Reactive Substances and Glutathione reductase activity methods. The exercise decreased lipid peroxidation and increased glutathione reductase activity in the substantia nigra. In contrast, in the striatum, exercise increased lipid peroxidation and decreased glutathione reductase activity. Exercise increased contralateral rotations and reduces immobility levels at 14 days post lesion. The exercise prior to 6-OHDA lesion had protective action only in substantia nigra against oxidative stress.

Keywords

Parkinson disease Exercise therapy Movement disorders Oxidative stress 

Notes

Acknowledgements

To Elizabeth Sumi Yamada, Anderson Valente Amaral e Riky Douglas Gomes for helping the researchers with the manuscript translation.

Funding information

This study was funded by Fundação Amazônia de Amparo a Estudos e Pesquisas (FAPESPA) by the technical cooperation agreement n° 003/2014-FAPESPA/UEPA.

Compliance with ethical standards

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. (University of Pará State’s Ethics Committee - protocol number 39/14).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Leonam Oliver Durval Oliveira
    • 1
    Email author
  • Pedro Iuri Castro da Silva
    • 1
  • Renato Pereira Rodrigues Filho
    • 1
  • Raphaely Cristiny Sanches Progênio
    • 1
  • Victor Douglas Pereira Silva de Oliveira
    • 1
  • Renata Cunha Silva
    • 1
  • Mariseth Carvalho de Andrade
    • 1
  • Jofre Jacob da Silva Freitas
    • 2
  • Katia Simone Kietzer
    • 2
  1. 1.Laboratory of Morphophysiology Applied to HealthUniversity of Pará StateBelémBrazil
  2. 2.Morphology and Physiological Sciences DepartmentUniversity of Pará StateBelémBrazil

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