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Metabolic Brain Disease

, Volume 34, Issue 6, pp 1615–1627 | Cite as

Neuroprotective effects of some seaweeds against Zn – induced neuronal damage in HT-22 cells via modulation of redox imbalance, inhibition of apoptosis and acetylcholinesterase activity

  • Tosin A. OlasehindeEmail author
  • Ademola O. Olaniran
  • Anthony I. Okoh
Original Article

Abstract

Zinc plays an important role in neuronal signaling and neurotransmission. However, dyshomeostasis of this metal or its accumulation in the brain has been linked with neurological disorders such as Alzheimer’s disease and Parkinson’s disease. In this study, the neuroprotective effects of Ecklonia maxima (KPM), Gracilaria gracilis (GCL), Ulva lactuca (ULT) and Gelidium pristoides (MNP) in Zn –induced neurotoxicity in HT-22 cells was examined. Cells were treated with Zinc sulphate and/or aqueous - ethanol extracts and cell viability, apoptosis, acetylcholinesterase activity, including some antioxidant enzymes (catalase and superoxide dismutase activity) and glutathione (GSH) levels were determined. Malondialdehyde and nitric oxide levels produced in the Zn and/or seaweed extract treated cells were also determined. Prior treatment with the seaweed extracts improved cell viability and inhibited Zn – induced cell death. Acetylcholinesterase activity was significantly high in Zn treated cells compared to the control. Pre-treatment with the seaweed extracts triggered a decrease in acetylcholinesterase activity in Zn – treated cells. Furthermore, treatment with Zn caused a significant reduction in GSH levels as well as a decrease in superoxide dismutase and catalase activities. In contrast, the seaweed extract increased antioxidant enzyme activities and GSH levels. An increase in malondialdehyde and nitric oxide levels was also reversed after treatment with the seaweed extracts. These results suggest that the seaweed extracts improved cholinergic transmission disrupted by Zn – induced neurotoxicity and protected the cells against oxidative damage and neuroinflammation. The neuroprotective effects of the seaweed extracts could be linked to their bioactive constituents. Hence these seaweeds are potential sources of active ingredients with neuroprotective potentials and could be used for the development of functional foods and/or nutraceuticals.

Keywords

Neurodegeneration Seaweeds Acetylcholinesterase Zinc Oxidative stress 

Notes

Acknowledgements

Authors acknowledge the support of South African Medical Research Council and National Research Foundation of South Africa/The World Academy of Science.

Compliance with ethical standards

Conflict of interest

Authors here declare no conflict of interest

Supplementary material

11011_2019_469_MOESM1_ESM.pdf (280 kb)
ESM 1 (PDF 279 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and MicrobiologyUniversity of Fort HareAliceSouth Africa
  2. 2.SAMRC Microbial Water Quality Monitoring CentreUniversity of Fort HareAliceSouth Africa
  3. 3.Nutrition and Toxicology Division, Department of Food TechnologyFederal Institute of Industrial Research OshodiLagosNigeria
  4. 4.Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and ScienceUniversity of Kwazulu-NatalDurbanSouth Africa

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