Metabolic Brain Disease

, Volume 33, Issue 5, pp 1525–1531 | Cite as

Improving cognitive task in kindled rats by using low frequency stimulation during epileptogenesis

  • Zohreh GhotbeddinEmail author
  • Ahmad Ali Moazedi
  • Ali Yadollahpour
  • Faezeh Rendi
  • Mostafa Jalilifar
Original Article


Numerous studies indicate that one of the bad effects of epilepsy is cognitive impairment. In this study we focused on the effect of LFS as a potential anticonvulsant agent, during epileptogenesis on cognitive impairments induced by amygdala kindling in rat. Twenty-one adult rats were divided into 3 groups including control (n = 7), kindled (n = 7), and Kindled+LFS (KLFS) (n = 7). Animals in the kindled group received kindling stimulation in a rapid kindling manner (a 3 s train of 50 Hz monophasic pulses of 1 ms duration, 12 times a day) in amygdala whereas control animals had no stimulation. Four packages of LFS (each package consisting of 200 monophasic square pulses, 0.1 ms pulse duration at 1 Hz) were applied daily after termination of kindling stimulation in KLFS group. Spatial memory of all animals was tested using radial arm maze after termination of stimulation on acquisition trial days and 14 days after the final acquisition trial test. Epileptogenesis process significantly increased working and reference memory error compared to control groups whereas application of LFS immediately after kindling stimulation prevented this impairment in 8 arm radial maze and there was no significant difference between KLS and control groups. Our results indicated that application of LFS during kindling acquisition suppresses memory impairment in epileptogenesis by kindling stimulation.


Electrical kindling Low frequency stimulation Working memory Reference memory 8 arm radial maze Rat 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Zohreh Ghotbeddin
    • 1
    • 2
    Email author
  • Ahmad Ali Moazedi
    • 3
  • Ali Yadollahpour
    • 4
  • Faezeh Rendi
    • 5
  • Mostafa Jalilifar
    • 4
  1. 1.Department of Physiology, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran
  2. 2.Stem Cell and Transgenic Technology Research CenterShahid Chamran University of AhvazAhvazIran
  3. 3.Department of Biology, Faculty of ScienceShahid Chamran University of AhvazAhvazIran
  4. 4.Department of Medical Physics, School of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran
  5. 5.Department of Basic Sciences, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran

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