Telmisartan attenuates hydrogen peroxide-induced apoptosis in differentiated PC12 cells
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The present study investigated the protective actions of telmisartan, an angiotensin II type 1 receptor blocker (ARBs), against the cell apoptosis induced by exposure to hydrogen peroxide (H2O2) in differentiated PC12 cells. Preincubation of PC12 cells with telmisartan prevented H2O2-induced cytotoxicity as indicated by increased MTT (3,(4,5-dimethylthiazole-2-yl)2,5-diphenyl-tetrazolium bromide) reduction, decreased lactate dehydrogenase (LDH) release, and improved morphological changes. Hoechst 33,258 staining showed that telmisartan markedly reduced shrunken nuclei of the cells, and Western blot analysis indicated that telmisartan significantly attenuated caspase-3 activity, as indicated by decreased ratio of cleaved Caspase-3 to its precursor and increased ratio of Bcl-2/Bax. The present findings showed that telmisartan protected against cellular oxidative damages by inhibiting apoptotic response.
KeywordsTelmisartan Oxidative stress Cytotoxicity Apoptosis
This work was supported by grants from the National Natural Science Foundation of China (81573413 and 81773714 to Hao Hong) and the Fundamental Research Funds for the Central Universities (2632017PT01).
Compliance with ethical standards
Conflict of interest
The authors have no conflicts of interest.
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