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Metabolic Brain Disease

, Volume 33, Issue 4, pp 1327–1334 | Cite as

Telmisartan attenuates hydrogen peroxide-induced apoptosis in differentiated PC12 cells

  • Guan Tao Du
  • Xuan Ke
  • Guo Liang Meng
  • Guang Jun Liu
  • Hui Ying Wu
  • Jin Hong Gong
  • Xiao Dan Qian
  • Jin Luo Cheng
  • Hao Hong
Original Article

Abstract

The present study investigated the protective actions of telmisartan, an angiotensin II type 1 receptor blocker (ARBs), against the cell apoptosis induced by exposure to hydrogen peroxide (H2O2) in differentiated PC12 cells. Preincubation of PC12 cells with telmisartan prevented H2O2-induced cytotoxicity as indicated by increased MTT (3,(4,5-dimethylthiazole-2-yl)2,5-diphenyl-tetrazolium bromide) reduction, decreased lactate dehydrogenase (LDH) release, and improved morphological changes. Hoechst 33,258 staining showed that telmisartan markedly reduced shrunken nuclei of the cells, and Western blot analysis indicated that telmisartan significantly attenuated caspase-3 activity, as indicated by decreased ratio of cleaved Caspase-3 to its precursor and increased ratio of Bcl-2/Bax. The present findings showed that telmisartan protected against cellular oxidative damages by inhibiting apoptotic response.

Keywords

Telmisartan Oxidative stress Cytotoxicity Apoptosis 

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (81573413 and 81773714 to Hao Hong) and the Fundamental Research Funds for the Central Universities (2632017PT01).

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Guan Tao Du
    • 1
  • Xuan Ke
    • 2
  • Guo Liang Meng
    • 3
  • Guang Jun Liu
    • 1
  • Hui Ying Wu
    • 1
  • Jin Hong Gong
    • 1
  • Xiao Dan Qian
    • 1
  • Jin Luo Cheng
    • 1
  • Hao Hong
    • 2
  1. 1.Department of Pharmacy, Department of EndocrinologyChangzhou No. 2 People’s Hospital Affiliated with Nanjing Medical UniversityChangzhouChina
  2. 2.Department of PharmacologyChina Pharmaceutical UniversityNanjingChina
  3. 3.School of PharmacyNantong UniversityNantongChina

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