Protective effects of Scutellaria baicalensis Georgi extract on D-galactose induced aging rats
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Scutellaria baicalensis Georgi (SBG), a traditional Chinese herb, has attracted considerable attention for its wide range of pharmacological activities. This study aimed to investigate the intervention effects of SBG ethanol extract on aging rats induced by D-galactose (D-gal) and to explore potential mechanisms by serum and liver metabolic profiles. The aging rats were induced by the D-gal (100 mg/kg) for 10 weeks continuously with subcutaneous injection, while the control rats received physiological saline. Two other groups of rats were administered with 100 mg/kg/day and 200 mg/kg/day of SBG by oral route following D-gal injections. The abilities of spatial and learning memory were evaluated by open-field test and Morris water maze test. Then, some biochemical indexes related to cognitive ability and aging were measured. Histopathological feature in hippocampal region was observed by Hematoxylin and eosin (HE) staining. The changes of metabolic profiles were evaluated using proton nuclear magnetic resonance (1H NMR) spectroscopy coupled with multivariate data analysis. Results showed that SBG could significantly improve the learning and memory functions, reducing oxidative damage and histological abnormalities of hippocampus neurons. In addition, significant differences in the metabolic profiles were observed both in serum and liver between the model group and the control group. After the treatment using SBG, the levels of these metabolites are significantly changed back to their similar levels in the control group. These metabolic changes are related to the disturbance in amino acid metabolism, glycometabolism and choline metabolism. Hence, SBG may have the potential to improve neurodegeneration and provide brain protection.
KeywordsScutellaria baicalensis Aging D-galactose Metabolomics
We acknowledge financial supports from the Applied basic research project of Shanxi Province (No.201601D021164), the Innovation project of higher education institutions in Shanxi Province (No.2016120), the Construction of the Science and Technology Basic Condition Platform of Shanxi Province (No. 2014091022), and the Program of Science and Technology of Shanxi Province (No. 20140313008-14).
Compliance with ethical standards
Conflict of interest
The authors have declared that no conflict of interest exists.
- Baranyi A, Amouzadeh-Ghadikolai O, von Lewinski D, Rothenhäusler HB, Theokas S, Robier C, Mangge H, Reicht G, Hlade P, Meinitzer A (2016) Branched-chain amino acids as new biomarkers of major depression - a novel neurobiology of mood disorder. PLoS One 11:e0160542. https://doi.org/10.1371/journal.pone.0160542 CrossRefPubMedPubMedCentralGoogle Scholar
- Bouatra S, Aziat F, Mandal R, Guo AC, Wilson MR, Knox C, Bjorndahl TC, Krishnamurthy R, Saleem F, Liu P, Dame ZT, Poelzer J, Huynh J, Yallou FS, Psychogios N, Dong E, Bogumil R, Roehring C, Wishart DS (2013) The human urine metabolome. PLoS One 8(9):e73076. https://doi.org/10.1371/journal.pone.0073076 CrossRefPubMedPubMedCentralGoogle Scholar
- Budni J, Pacheco R, da Silva S, Garcez ML, Mina F, Bellettini-Santos T, de Medeiros J, Voss BC, Steckert AV, Valvassori Sda S, Quevedo J (2016) Oral administration of d-galactose induces cognitive impairments and oxidative damage in rats. Behav Brain Res 302:35–43. https://doi.org/10.1016/j.bbr.2015.12.041 CrossRefPubMedGoogle Scholar
- Chaung HC, Chang CD, Chen PH, Chang CJ, Liu SH, Chen CC (2013) Docosahexaenoic acid and phosphatidylserine improves the antioxidant activities in vitro and in vivo and cognitive functions of the developing brain. Food Chem 138:342–347. https://doi.org/10.1016/j.foodchem.2012.10.082 CrossRefPubMedGoogle Scholar
- Chiu CY, Yeh KW, Lin G, Chiang MH, Yang SC, Chao WJ, Yao TC, Tsai MH, Hua MC, Liao SL, Lai SH, Cheng ML, Huang JL (2016) Metabolomics reveals dynamic metabolic changes associated with age in early childhood. PLoS One 11:e0149823. https://doi.org/10.1371/journal.pone.0149823 CrossRefPubMedPubMedCentralGoogle Scholar
- Chu H, Zhang A, Han Y, Lu S, Kong L, Han J, Liu Z, Sun H, Wang X (2016) Metabolomics approach to explore the effects of Kai-Xin-san on Alzheimer's disease using UPLC/ESI-Q-TOF mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 1015-1016:50–61. https://doi.org/10.1016/j.jchromb.2016.02.007 CrossRefPubMedGoogle Scholar
- Haider S, Saleem S, Perveen T, Tabassum S, Batool Z, Sadir S, Liaquat L, Madiha S (2014) Age-related learning and memory deficits in rats: role of altered brain neurotransmitters, acetylcholinesterase activity and changes in antioxidant defense system. Age (Dordr) 36:9653. https://doi.org/10.1007/s11357-014-9653-0 CrossRefGoogle Scholar
- Haider S, Liaquat L, Shahzad S, Sadir S, Madiha S, Batool Z, Tabassum S, Saleem S, Naqvi F, Perveen T (2015) A high dose of short term exogenous D-galactose administration in young male rats produces symptoms simulating the natural aging process. Life Sci 124:110–119. https://doi.org/10.1016/j.lfs.2015.01.016 CrossRefPubMedGoogle Scholar
- Hwang YK, Jinhua M, Choi BR, Cui CA, Jeon WK, Kim H, Kim HY, Han SH, Han JS (2011) Effects of Scutellaria baicalensis on chronic cerebral hypoperfusion-induced memory impairments and chronic lipopolysaccharide infusion-induced memory impairments. J Ethnopharmacol 137:681–689. https://doi.org/10.1016/j.jep.2011.06.025 CrossRefPubMedGoogle Scholar
- Jeong K, Shin YC, Park S, Park JS, Kim N, Um JY, Go H, Sun S, Lee S, Park W, Choi Y, Song Y, Kim G, Jeon C, Park J, Lee K, Bang O, Ko SG (2011) Ethanol extract of Scutellaria baicalensis Georgi prevents oxidative damage and neuroinflammation and memorial impairments in artificial senescense mice. J Biomed Sci 18:14. https://doi.org/10.1186/1423-0127-18-14 CrossRefPubMedPubMedCentralGoogle Scholar
- Kojima-Yuasa A, Yamamoto T, Yaku K, Hirota S, Takenaka S, Kawabe K, Matsui-Yuasa I (2016) 1'-Acetoxychavicol acetate ameliorates age-related spatial memory deterioration by increasing serum ketone body production as a complementary energy source for neuronal cells. Chem Biol Interact 257:101–109. https://doi.org/10.1016/j.cbi.2016.07.031 CrossRefPubMedGoogle Scholar
- Tomassini A, Vitalone A, Marini F, Praticò G, Sciubba F, Bevilacqua M, Delfini M, Di Sotto A, Di Giacomo S, Mariani P, Mammola CL, Gaudio E, Miccheli A, Mazzanti G (2014) 1H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat. J Proteome Res 13:5848–5859. https://doi.org/10.1021/pr500748r CrossRefPubMedGoogle Scholar
- Wang T, Di G, Yang L, Dun Y, Sun Z, Wan J, Peng B, Liu C, Xiong G, Zhang C, Yuan D (2015a) Saponins from Panax japonicus attenuate D-galactose-induced cognitive impairment through its anti-oxidative and anti-apoptotic effects in rats. J Pharm Pharmacol 67:1284–1296. https://doi.org/10.1111/jphp.12413 CrossRefPubMedGoogle Scholar
- Xie S, Zhou W, Tian L, Niu J, Liu Y (2016) Effect of N-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, anti-oxidative and immune ability of Nile tilapia, Oreochromis niloticus. Fish Shellfish Immunol 55:233–241. https://doi.org/10.1016/j.fsi.2016.05.033 CrossRefPubMedGoogle Scholar
- Xu LQ, Xie YL, Gui SH, Zhang X, Mo ZZ, Sun CY, Li CL, Luo DD, Zhang ZB, Su ZR, Xie JH (2016) Polydatin attenuates d-galactose-induced liver and brain damage through its anti-oxidative, anti-inflammatory and anti-apoptotic effects in mice. Food Funct 7:4545–4555. https://doi.org/10.1039/c6fo01057a CrossRefPubMedGoogle Scholar