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Metabolic Brain Disease

, Volume 33, Issue 1, pp 79–88 | Cite as

The neuroprotective effects of hydro-alcoholic extract of olive (Olea europaea L.) leaf on rotenone-induced Parkinson’s disease in rat

  • Maryam SarbishegiEmail author
  • Enam Alhagh Charkhat GorgichEmail author
  • Ozra Khajavi
  • Gholamreza Komeili
  • Saeedeh Salimi
Original Article

Abstract

Parkinson’s disease (PD) is an age-related disease in which dopaminergic neurons in the nigrostriatal pathway are destroyed, resulting in movement and behavioral problems. Oxidative stress and the generation of reactive oxygen species play key roles in neurodegenerative diseases, such as PD. Rotenone (ROT) is a common pesticide that induces oxidative stress. Olive leaves extract (OLE) has antioxidant and neuroprotective effects. Thus, the aim of this study was to investigate the neuroprotective effects of OLE on ROT-induced oxidative stress in the midbrain of a rat model of PD. Ninety-six Wistar rats were randomly divided into the following 6 groups (n = 16 rats/group): Control, Sham, ROT, and 3 ROT + OLE (75, 150, and 300 mg/kg/daily) groups. ROT (2.5 mg/kg/48 h) was injected subcutaneously, and vehicle or OLE was orally administered for 30 days. The animals were then sacrificed, and their brains were removed. Biochemical measures, including the levels of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and malondialdehyde (MDA), and the number of tyrosine hydroxylase (TH)-positive neurons were determined, and behavioral (rotarod and hanging) tests were conducted. The balance and muscle strength of the OLE (150 and 300 mg/kg)-treated groups were significantly improved. Treatment with OLE prevented the increases in the levels of MDA, significantly improved the SOD, CAT, and GPx levels in the midbrain, and prevented the depletion of the TH-positive neurons. These findings suggested that OLE has neuroprotective properties and that it might be useful for preventing the death of dopaminergic neurons in patients with PD.

Keywords

Parkinson’s disease Olive leaves extract Rotenone Oxidative stress 

Notes

Acknowledgements

The authors would like to express their appreciation for the support of the deputy vice-chancellor of research of ZAUMS and the Head of the Department of Histology.

Author’s contribution

M. Sarbishegi designed and supervised the study, analyzed the data, and wrote the manuscript; EA. Charkhat Gorgich assisted with the modeling and writing of the manuscript. Ozra Khajavi assisted with the modeling and provided technical assistance. Gh. Komeili supervised the behavioral tests. S. Salimi supervised the biochemical analyses. This study was funded by the deputy vice-chancellor of research of ZAUMS (research project No. 7712).

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Copyright information

© Springer Science+Business Media, LLC 2017
Corrected publication October/2017

Authors and Affiliations

  • Maryam Sarbishegi
    • 1
    • 2
    Email author
  • Enam Alhagh Charkhat Gorgich
    • 3
    Email author
  • Ozra Khajavi
    • 2
  • Gholamreza Komeili
    • 4
  • Saeedeh Salimi
    • 1
    • 5
  1. 1.Cellular and Molecular Research CenterZahedan University of Medical SciencesZahedanIran
  2. 2.Department of Anatomy, School of MedicineZahedan University of Medical SciencesZahedanIran
  3. 3.Department of Histology, School of MedicineZahedan University of Medical SciencesZahedanIran
  4. 4.Department of Physiology, School of MedicineZahedan University of Medical SciencesZahedanIran
  5. 5.Department of Biochemistry, School of MedicineZahedan University of Medical SciencesZahedanIran

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