Metabolic Brain Disease

, Volume 31, Issue 6, pp 1455–1461 | Cite as

Timosaponin B-II ameliorates scopolamine-induced cognition deficits by attenuating acetylcholinesterase activity and brain oxidative damage in mice

  • Xu Zhao
  • Chunmei Liu
  • Yu Qi
  • Lina Fang
  • Jie Luo
  • Kaishun Bi
  • Ying Jia
Original Article


Timosaponin B-II (TB-II) is a main active saponin isolated from the rhizome of Anemarrhena asphodeloides Bge., which is widely used in traditional Chinese medicine. In this study, the effect of TB-II on learning and memory was investigated in a scopolamine-induced mouse model of Alzheimer’s disease. The results of behavioral tests indicated that TB-II significantly increased the spontaneous alternation in the Y-maze test, and reversed the shortening of step-through latency induced by scopolamine in the passive avoidance test, showing protective effects on short-term and working memory. In the Morris water maze test, TB-II reduced the escape latency time in the training trial, and increased the swimming time in the target quadrant in the probe trial. Biochemical data demonstrated that TB-II significantly inhibited acetylcholinesterase (AChE) activity in the cerebral cortex and hippocampus of mice. Moreover, TB-II markably attenuated the reduction in glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, and decreased malondialdehyde (MDA) levels, which are key biomarkers of brain oxidative stress. These results indicated that TB-II offers protection against scopolamine-induced deficits in learning and memory, possibly by inhibiting AChE and preventing oxidative stress damage. The findings suggested that TB-II has a potential therapeutic effect on cognitive and behavioral impairment.


Timosaponin B-II Alzheimer’s disease Learning and memory Acetylcholinesterase Oxidative stress 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Xu Zhao
    • 1
  • Chunmei Liu
    • 2
  • Yu Qi
    • 2
  • Lina Fang
    • 3
  • Jie Luo
    • 2
  • Kaishun Bi
    • 4
  • Ying Jia
    • 1
  1. 1.School of Functional Food and WineShenyang Pharmaceutical UniversityShenyangPeople’s Republic of China
  2. 2.School of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyangChina
  3. 3.School of Basic Medical SciencesShenyang Medical CollegeShenyangChina
  4. 4.School of PharmacyShenyang Pharmaceutical UniversityShenyangChina

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