Metabolic Brain Disease

, Volume 31, Issue 2, pp 395–404 | Cite as

Satureja bachtiarica ameliorate beta-amyloid induced memory impairment, oxidative stress and cholinergic deficit in animal model of Alzheimer’s disease

  • Maliheh Soodi
  • Soodabeh Saeidnia
  • Mohammad Sharifzadeh
  • Homa Hajimehdipoor
  • Abolfazl Dashti
  • Mohammad Reza Sepand
  • Shahla Moradi
Original Article

Abstract

Extracellular deposition of Beta-amyloid peptide (Aβ) is the main finding in the pathophysiology of Alzheimer’s disease (AD), which damages cholinergic neurons through oxidative stress and reduces the cholinergic neurotransmission. Satureja bachtiarica is a medicinal plant from the Lamiaceae family which was widely used in Iranian traditional medicine. The aim of the present study was to investigate possible protective effects of S. bachtiarica methanolic extract on Aβ induced spatial memory impairment in Morris Water Maze (MWM), oxidative stress and cholinergic neuron degeneration. Pre- aggregated Aβ was injected into the hippocampus of each rat bilaterally (10 μg/rat) and MWM task was performed 14 days later to evaluate learning and memory function. Methanolic extract of S.bachtiarica (10, 50 and 100 mg/Kg) was injected intraperitoneally for 19 consecutive days, after Aβ injection. After the probe test the brain tissue were collected and lipid peroxidation, Acetylcholinesterase (AChE) activity and Cholin Acetyl Transferees (ChAT) immunorectivity were measured in the hippocampus. Intrahipocampal injection of Aβ impaired learning and memory in MWM in training days and probe trail. Methanolic extract of S. bachtiarica (50 and 100 mg/Kg) could attenuate Aβ-induced memory deficit. ChAT immunostaining revealed that cholinergic neurons were loss in Aβ- injected group and S. bachtiarica (100 mg/Kg) could ameliorate Aβ- induced ChAT reduction in the hippocampus. Also S. bachtiarica could ameliorate Aβ-induced lipid peroxidation and AChE activity increase in the hippocampus. In conclusion our study represent that S.bachtiarica methanolic extract can improve Aβ-induced memory impairment and cholinergic loss then we recommended this extract as a candidate for further investigation in treatment of AD.

Keywords

Alzheimer’s disease Beta amyloid peptide Satureja bachtiarica ChAT Oxidative stress Memory 

Notes

Acknowledgments

The Author wish to thank Tehran University of Medical Sciences and Health Services Grant No. 9108.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Maliheh Soodi
    • 1
  • Soodabeh Saeidnia
    • 2
  • Mohammad Sharifzadeh
    • 3
  • Homa Hajimehdipoor
    • 4
  • Abolfazl Dashti
    • 1
  • Mohammad Reza Sepand
    • 3
  • Shahla Moradi
    • 1
  1. 1.Department of Toxicology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  2. 2.Medicinal Plants Research Center, Faculty of PharmacyTehran University of Medical SciencesTehranIran
  3. 3.Department of Pharmacology and Toxicology, Faculty of PharmacyTehran University of Medical SciencesTehranIran
  4. 4.Department of Traditional Pharmacy, School of Traditional MedicineShahid Beheshti University of Medical SciencesTehranIran

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