Metabolic Brain Disease

, Volume 28, Issue 4, pp 563–570 | Cite as

Brain effect of insulin and clonazepam in diabetic rats under depressive-like behavior

  • Carlos Alberto Yasin Wayhs
  • Caroline Paula Mescka
  • Camila Simioni Vanzin
  • Graziela Schmitt Ribas
  • Gilian Guerreiro
  • Maurício Schüler Nin
  • Vanusa Manfredini
  • Helena Maria Tannhauser Barros
  • Carmen Regla Vargas
Original Paper


Diabetes mellitus is characterized by hyperglycemia resulting from defects on insulin secretion, insulin action, or both. It has recently become clear that the central nervous system is not spared from the deleterious effects of diabetes, since diabetic encephalopathy was recognized as a complication of this heterogeneous metabolic disorder. There is a well recognized association between depression and diabetes, once prevalence of depression in diabetic patients is higher than in general population, and clonazepam is being used to treat this complication. Oxidative stress is widely accepted as playing a key mediatory role in the development and progression of diabetes and its complications. In this work we analyzed DNA damage by comet assay and lipid damage in prefrontal cortex, hippocampus and striatum of streptozotocin-induced diabetic rats submitted to the forced swimming test. It was verified that the diabetic group presented DNA and lipid damage in the brain areas evaluated, when compared to the control groups. Additionally, a significant reduction of the DNA and lipid damage in animals treated with insulin and/or clonazepam was observed. These data suggest that the association of these two drugs could protect against DNA and lipid damage in diabetic rats submitted to the forced swimming test, an animal model of depression.


Brain Comet assay Depression Diabetes Lipid damage Oxidative stress 



We thank Mario Serapião for his technical support. We also appreciate the financial support from Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundo de Incentivo à Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre (FIPE/HCPA). CRV is the recipient of a CNPq 2 Researcher Productivity Grant. HMTB is the recipient of a CNPq 1C Researcher Productivity Grant, and CAYW and CPM are recipients of fellowships from the CNPq or CAPES.

Conflict of interest

The authors declare that there are no conflicts of interest including any financial, personal or other relationships with other people or organizations.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Carlos Alberto Yasin Wayhs
    • 1
    • 2
  • Caroline Paula Mescka
    • 2
    • 3
  • Camila Simioni Vanzin
    • 2
    • 3
  • Graziela Schmitt Ribas
    • 2
  • Gilian Guerreiro
    • 2
    • 4
  • Maurício Schüler Nin
    • 5
  • Vanusa Manfredini
    • 6
  • Helena Maria Tannhauser Barros
    • 5
  • Carmen Regla Vargas
    • 1
    • 2
    • 3
  1. 1.Programa de Pós-Graduação em Ciências FarmacêuticasUFRGSPorto AlegreBrazil
  2. 2.Serviço de Genética MédicaHCPAPorto AlegreBrazil
  3. 3.Programa de Pós-Graduação em Ciências Biológicas: BioquímicaUFRGSPorto AlegreBrazil
  4. 4.Faculdade de FarmáciaUFRGSPorto AlegreBrazil
  5. 5.Departamento de Ciências Básicas da Saúde, Disciplina de FarmacologiaUniversidade Federal de Ciências da Saúde de Porto Alegre, UFCSPAPorto AlegreBrazil
  6. 6.Programa de Pós-Graduação em BioquímicaUNIPAMPAUruguaianaBrazil

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