Molecular and Cellular Biochemistry

, Volume 465, Issue 1–2, pp 141–153 | Cite as

Role of alpha-melanocyte stimulating hormone (α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress

  • Renuka ChoudharyEmail author
  • Anil Sharma
  • Sudarshan KumarEmail author
  • Ramesh Chandra Upadhyay
  • Sohan Vir Singh
  • Ashok Mohanty


Ultraviolet radiations (UVR) are responsible for a wide variety of acute and chronic effects on the animal skin. However, the effect of UVR-induced oxidative stress and protection through paracrine factors on animal skin has received little attention. We previously demonstrated how heat stress-induced adaptation in Bos indicus melanocytes was dependent on the level of melanin and reduction of apoptosis. Therefore, in the present investigation, the survival mechanisms adopted by melanocytes under UV stress and the role of α-MSH in cell survival under in vitro conditions were studied. After the treatment of melanocyte cells with UVR (using Osram ultravitalux 300 W lamp), analysis of Gene expression using Real-Time PCR was done to study the adopted molecular pathways under stressful conditions. In addition, α-MSH was used to assess its modulating role in cell survival under stress. This study revealed the increase in the expression of genes related to melanogenesis, cell cycle, heat shock proteins, and apoptosis of the cells after UVR stress and demonstrated the role of paracrine factor (α-MSH) in elevating the protection response to stressful conditions like UVR stress by increasing the melanogenesis and decreasing the mitochondrial-mediated apoptosis. Based on the results of the present study, it can be stated that α-MSH can play a pivotal role in the protection of animal skin cells under stressful conditions in climate-changing scenario.


α-MSH Apoptosis Bos indicus Melanocyte UVR stress 



The authors express sincere thanks to the Director, NDRI, Karnal for providing necessary facilities for conducting the research. The financial assistance for the research work was provided from National Innovations on Climate Resilient Agriculture (NICRA project Grant No-2049/3033) of Indian Council of Agricultural Research (ICAR), New Delhi and is highly acknowledged.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

Supplementary material

11010_2019_3674_MOESM1_ESM.docx (18 kb)
Electronic supplementary material 1 (DOCX 18 kb)
11010_2019_3674_MOESM2_ESM.docx (11 kb)
Electronic supplementary material 2 (DOCX 11 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Renuka Choudhary
    • 1
    • 2
    • 4
    Email author
  • Anil Sharma
    • 2
  • Sudarshan Kumar
    • 3
    Email author
  • Ramesh Chandra Upadhyay
    • 1
  • Sohan Vir Singh
    • 1
  • Ashok Mohanty
    • 3
  1. 1.Climate Resilient Livestock Research CentreNational Dairy Research InstituteKarnalIndia
  2. 2.Department of BiotechnologyMaharishi Markandeshwar Deemed to be UniversityMullanaIndia
  3. 3.Animal Biotechnology CentreNational Dairy Research InstituteKarnalIndia
  4. 4.Department of BiotechnologyMaharishi Markandeshwar Deemed to be UniversityAmbalaIndia

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