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Molecular and Cellular Biochemistry

, Volume 465, Issue 1–2, pp 141–153 | Cite as

Role of alpha-melanocyte stimulating hormone (α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress

  • Renuka ChoudharyEmail author
  • Anil Sharma
  • Sudarshan KumarEmail author
  • Ramesh Chandra Upadhyay
  • Sohan Vir Singh
  • Ashok Mohanty
Article
  • 34 Downloads

Abstract

Ultraviolet radiations (UVR) are responsible for a wide variety of acute and chronic effects on the animal skin. However, the effect of UVR-induced oxidative stress and protection through paracrine factors on animal skin has received little attention. We previously demonstrated how heat stress-induced adaptation in Bos indicus melanocytes was dependent on the level of melanin and reduction of apoptosis. Therefore, in the present investigation, the survival mechanisms adopted by melanocytes under UV stress and the role of α-MSH in cell survival under in vitro conditions were studied. After the treatment of melanocyte cells with UVR (using Osram ultravitalux 300 W lamp), analysis of Gene expression using Real-Time PCR was done to study the adopted molecular pathways under stressful conditions. In addition, α-MSH was used to assess its modulating role in cell survival under stress. This study revealed the increase in the expression of genes related to melanogenesis, cell cycle, heat shock proteins, and apoptosis of the cells after UVR stress and demonstrated the role of paracrine factor (α-MSH) in elevating the protection response to stressful conditions like UVR stress by increasing the melanogenesis and decreasing the mitochondrial-mediated apoptosis. Based on the results of the present study, it can be stated that α-MSH can play a pivotal role in the protection of animal skin cells under stressful conditions in climate-changing scenario.

Keywords

α-MSH Apoptosis Bos indicus Melanocyte UVR stress 

Notes

Acknowledgements

The authors express sincere thanks to the Director, NDRI, Karnal for providing necessary facilities for conducting the research. The financial assistance for the research work was provided from National Innovations on Climate Resilient Agriculture (NICRA project Grant No-2049/3033) of Indian Council of Agricultural Research (ICAR), New Delhi and is highly acknowledged.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

Supplementary material

11010_2019_3674_MOESM1_ESM.docx (18 kb)
Electronic supplementary material 1 (DOCX 18 kb)
11010_2019_3674_MOESM2_ESM.docx (11 kb)
Electronic supplementary material 2 (DOCX 11 kb)

References

  1. 1.
    Silanikove N, Koluman N (2015) Impact of climate change on the dairy industry in temperate zones: predications on the overall negative impact and on the positive role of dairy goats in adaptation to earth warming. Small Rumin Res. 123:27–34CrossRefGoogle Scholar
  2. 2.
    Gilchrest BA, Eller MS, Geller AC, Yaar M (1999) The pathogenesis of melanoma induced by ultraviolet radiation. N Engl J Med 340:1341–1348.  https://doi.org/10.1056/NEJM199904293401707 CrossRefPubMedGoogle Scholar
  3. 3.
    De Fabo EC, Noonan FP, Fears T, Merlino G (2004) Ultraviolet B but not ultraviolet A radiation initiates melanoma. Cancer Res 64:6372–6376.  https://doi.org/10.1158/0008-5472.CAN-04-1454 CrossRefPubMedGoogle Scholar
  4. 4.
    Collier RJ, Collier JL (2012) Environmental physiology of livestock. Wiley, New YorkCrossRefGoogle Scholar
  5. 5.
    Stephen ID, Coetzee V, Perrett DI (2011) Carotenoid and melanin pigment coloration affect perceived human health. Evol Hum Behav 32:216–227CrossRefGoogle Scholar
  6. 6.
    Simon JD, Peles D, Wakamatsu K, Ito S (2009) Current challenges in understanding melanogenesis: bridging chemistry, biological control, morphology, and function. Pigment Cell Melanoma Res 22:563–579.  https://doi.org/10.1111/j.1755-148X.2009.00610.x CrossRefPubMedGoogle Scholar
  7. 7.
    Brenner M, Berking C (2010) Principles of skin pigmentation. Biochemistry and regulation of melanogenesis. Hautarzt 61:554–560.  https://doi.org/10.1007/s00105-009-1913-z CrossRefPubMedGoogle Scholar
  8. 8.
    Abdel-Malek ZA, Kadekaro AL, Swope VB (2010) Stepping up melanocytes to the challenge of UV exposure. Pigment Cell Melanoma Res 23:171–186.  https://doi.org/10.1111/j.1755-148X.2010.00679.x CrossRefPubMedGoogle Scholar
  9. 9.
    Hunt G, Kyne S, Wakamatsu K, Ito S, Thody AJ (1995) Nle4DPhe7 alpha-melanocyte-stimulating hormone increases the eumelanin:phaeomelanin ratio in cultured human melanocytes. J Invest Dermatol 104:83–85CrossRefGoogle Scholar
  10. 10.
    Thody AJ, Graham A (1998) Does alpha-MSH have a role in regulating skin pigmentation in humans? Pigment Cell Res 11:265–274CrossRefGoogle Scholar
  11. 11.
    Rees JL (2003) Genetics of hair and skin color. Annu Rev Genet 37:67–90.  https://doi.org/10.1146/annurev.genet.37.110801.143233 CrossRefPubMedGoogle Scholar
  12. 12.
    Kumar A, Ashraf S, Goud TS, Grewal A, Singh SV, Yadav BR, Upadhyay RC (2015) Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition. J Therm Biol 51:55–64.  https://doi.org/10.1016/j.jtherbio.2015.03.006 CrossRefPubMedGoogle Scholar
  13. 13.
    Maibam U, Singh SV, Singh AK, Kumar S, Upadhyay RC (2014) Expression of skin color genes in lymphocytes of Karan Fries cattle and seasonal relationship with tyrosinase and cortisol. Trop Anim Health Prod 46:1155–1160.  https://doi.org/10.1007/s11250-014-0620-7 CrossRefPubMedGoogle Scholar
  14. 14.
    Singh AK, Upadhyay RC, Malakar D, Kumar S, Singh SV (2014) Effect of thermal stress on HSP70 expression in dermal fibroblast of zebu (Tharparkar) and crossbred (Karan-Fries) cattle. J Therm Biol 43:46–53.  https://doi.org/10.1016/j.jtherbio.2014.04.006 CrossRefPubMedGoogle Scholar
  15. 15.
    Choudhary R, Goud TS, Kumar A, Sharma AK, Singh SV, Upadhyay RC, Mohanty AK, Kumar S (2017) Heat stress induced adaptation in melanocytes is dependent on the level of melanin and reduction of apoptosis. J Dermatol Sci 85:250–252.  https://doi.org/10.1016/j.jdermsci.2016.12.012 CrossRefPubMedGoogle Scholar
  16. 16.
    Freshney RI (1994) Culture of animal cells: a manual of basic technique. Wiley, New YorkGoogle Scholar
  17. 17.
    Choudhary R, Kumar S, Singh SV, Sharma AK, Goud TS, Srivastava AK, Kumar A, Mohanty AK, Upadhyay RC (2016) Validation of putative reference genes for gene expression studies in heat stressed and alpha-MSH treated melanocyte cells of Bos indicus using real-time quantitative PCR. Mol Cell Probes 30:161–167.  https://doi.org/10.1016/j.mcp.2016.03.002 CrossRefPubMedGoogle Scholar
  18. 18.
    Oancea E, Vriens J, Brauchi S, Jun J, Splawski I, Clapham DE (2009) TRPM1 forms ion channels associated with melanin content in melanocytes. Sci Signal 2:21.  https://doi.org/10.1126/scisignal.2000146 CrossRefGoogle Scholar
  19. 19.
    Amna T, Park KM, Cho IK, Choi TJ, Lee SS, Seo KS, Hwang I (2012) Substantial effect of melanin influencing factors on in vitro melanogenesis in muzzle melanocytes of differently colored hanwoo. Asian Australas J Anim Sci 25:1029–1037.  https://doi.org/10.5713/ajas.2011.11287 CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Bivik CA, Larsson PK, Kagedal KM, Rosdahl IK, Ollinger KM (2006) UVA/B-induced apoptosis in human melanocytes involves translocation of cathepsins and Bcl-2 family members. J Invest Dermatol 126:1119–1127.  https://doi.org/10.1038/sj.jid.5700124 CrossRefPubMedGoogle Scholar
  21. 21.
    Snell RS (1963) The effect of ultraviolet irradiation on melanogenesis. J Invest Dermatol 40:127–132CrossRefGoogle Scholar
  22. 22.
    Friedmann PS, Gilchrest BA (1987) Ultraviolet radiation directly induces pigment production by cultured human melanocytes. J Cell Physiol 133:88–94.  https://doi.org/10.1002/jcp.1041330111 CrossRefPubMedGoogle Scholar
  23. 23.
    Nakazawa K, Sahuc F, Damour O, Collombel C, Nakazawa H (1998) Regulatory effects of heat on normal human melanocyte growth and melanogenesis: comparative study with UVB. J Invest Dermatol 110:972–977.  https://doi.org/10.1046/j.1523-1747.1998.00204.x CrossRefPubMedGoogle Scholar
  24. 24.
    Snell RS (1965) An electron microscopic study of the dendritic cells in the basal layer of guinea-pig epidermis. Z Zellforsch Mikrosk Anat 66:457–470CrossRefGoogle Scholar
  25. 25.
    Quevedo WC Jr, Szabo G, Virks J, Sinesi SJ (1965) Melanocyte populations in UV-irradiated human skin. J Invest Dermatol 45:295–298CrossRefGoogle Scholar
  26. 26.
    Pathak MA, Sinesi SJ, Szabo G (1965) The effect of a single dose of ultraviolet radiation on epidermal melanocytes. J Invest Dermatol 45:520–528CrossRefGoogle Scholar
  27. 27.
    McCallion AS, Chakravarti A (2001) EDNRB/EDN3 and Hirschsprung disease type II. Pigment Cell Res 14:161–169CrossRefGoogle Scholar
  28. 28.
    Goding CR (2000) Melanocyte development and malignant melanoma. Forum (Genova) 10:176–187Google Scholar
  29. 29.
    Wehrle-Haller B (2003) The role of Kit-ligand in melanocyte development and epidermal homeostasis. Pigment Cell Res 16:287–296CrossRefGoogle Scholar
  30. 30.
    Matsuda M, Hoshino T, Yamashita Y, Tanaka K, Maji D, Sato K, Adachi H, Sobue G, Ihn H, Funasaka Y, Mizushima T (2010) Prevention of UVB radiation-induced epidermal damage by expression of heat shock protein 70. J Biol Chem 285:5848–5858.  https://doi.org/10.1074/jbc.M109.063453 CrossRefPubMedGoogle Scholar
  31. 31.
    Jarpe MB, Widmann C, Knall C, Schlesinger TK, Gibson S, Yujiri T, Fanger GR, Gelfand EW, Johnson GL (1998) Anti-apoptotic versus pro-apoptotic signal transduction: checkpoints and stop signs along the road to death. Oncogene 17:1475–1482.  https://doi.org/10.1038/sj.onc.1202183 CrossRefPubMedGoogle Scholar
  32. 32.
    Haycock JW, Rowe SJ, Cartledge S, Wyatt A, Ghanem G, Morandini R, Rennie IG, MacNeil S (2000) Alpha-melanocyte-stimulating hormone reduces impact of proinflammatory cytokine and peroxide-generated oxidative stress on keratinocyte and melanoma cell lines. J Biol Chem 275:15629–15636.  https://doi.org/10.1074/jbc.275.21.15629 CrossRefPubMedGoogle Scholar
  33. 33.
    Valverde P, Manning P, McNeil CJ, Thody AJ (1996) Activation of tyrosinase reduces the cytotoxic effects of the superoxide anion in B16 mouse melanoma cells. Pigment Cell Res 9:77–84CrossRefGoogle Scholar
  34. 34.
    Kadekaro AL, Kavanagh R, Kanto H, Terzieva S, Hauser J, Kobayashi N, Schwemberger S, Cornelius J, Babcock G, Shertzer HG, Scott G, Abdel-Malek ZA (2005) alpha-Melanocortin and endothelin-1 activate antiapoptotic pathways and reduce DNA damage in human melanocytes. Cancer Res 65:4292–4299.  https://doi.org/10.1158/0008-5472.CAN-04-4535 CrossRefPubMedGoogle Scholar
  35. 35.
    Kadekaro AL, Chen J, Yang J, Chen S, Jameson J, Swope VB, Cheng T, Kadakia M, Abdel-Malek Z (2012) Alpha-melanocyte-stimulating hormone suppresses oxidative stress through a p53-mediated signaling pathway in human melanocytes. Mol Cancer Res 10:778–786.  https://doi.org/10.1158/1541-7786.MCR-11-0436 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Renuka Choudhary
    • 1
    • 2
    • 4
    Email author
  • Anil Sharma
    • 2
  • Sudarshan Kumar
    • 3
    Email author
  • Ramesh Chandra Upadhyay
    • 1
  • Sohan Vir Singh
    • 1
  • Ashok Mohanty
    • 3
  1. 1.Climate Resilient Livestock Research CentreNational Dairy Research InstituteKarnalIndia
  2. 2.Department of BiotechnologyMaharishi Markandeshwar Deemed to be UniversityMullanaIndia
  3. 3.Animal Biotechnology CentreNational Dairy Research InstituteKarnalIndia
  4. 4.Department of BiotechnologyMaharishi Markandeshwar Deemed to be UniversityAmbalaIndia

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