Role of alpha-melanocyte stimulating hormone (α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress
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Ultraviolet radiations (UVR) are responsible for a wide variety of acute and chronic effects on the animal skin. However, the effect of UVR-induced oxidative stress and protection through paracrine factors on animal skin has received little attention. We previously demonstrated how heat stress-induced adaptation in Bos indicus melanocytes was dependent on the level of melanin and reduction of apoptosis. Therefore, in the present investigation, the survival mechanisms adopted by melanocytes under UV stress and the role of α-MSH in cell survival under in vitro conditions were studied. After the treatment of melanocyte cells with UVR (using Osram ultravitalux 300 W lamp), analysis of Gene expression using Real-Time PCR was done to study the adopted molecular pathways under stressful conditions. In addition, α-MSH was used to assess its modulating role in cell survival under stress. This study revealed the increase in the expression of genes related to melanogenesis, cell cycle, heat shock proteins, and apoptosis of the cells after UVR stress and demonstrated the role of paracrine factor (α-MSH) in elevating the protection response to stressful conditions like UVR stress by increasing the melanogenesis and decreasing the mitochondrial-mediated apoptosis. Based on the results of the present study, it can be stated that α-MSH can play a pivotal role in the protection of animal skin cells under stressful conditions in climate-changing scenario.
Keywordsα-MSH Apoptosis Bos indicus Melanocyte UVR stress
The authors express sincere thanks to the Director, NDRI, Karnal for providing necessary facilities for conducting the research. The financial assistance for the research work was provided from National Innovations on Climate Resilient Agriculture (NICRA project Grant No-2049/3033) of Indian Council of Agricultural Research (ICAR), New Delhi and is highly acknowledged.
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest to declare.
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