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Molecular and Cellular Biochemistry

, Volume 461, Issue 1–2, pp 141–150 | Cite as

Effects of different dietary regimes alone or in combination with standardized Aronia melanocarpa extract supplementation on lipid and fatty acids profiles in rats

  • Petar Milic
  • Jovana Jeremic
  • Vladimir Zivkovic
  • Ivan Srejovic
  • Nevena Jeremic
  • Jovana Bradic
  • Tamara Nikolic Turnic
  • Isidora Milosavljevic
  • Sergey Bolevich
  • Stefani Bolevich
  • Milica Labudovic Borovic
  • Aleksandra Arsic
  • Miroslav Mitrovic
  • Vladimir JakovljevicEmail author
  • Vesna Vucic
Article
  • 55 Downloads

Abstract

This study investigated different dietary strategies, high-fat (HFd), or standard diet (Sd) alone or in combination with standardized Aronia melanocarpa extract (SAE), as a polyphenol-rich diet, and their effects on lipids and fatty acids (FA) in rats with metabolic syndrome (MetS). Wistar albino rats were randomly divided into two groups: healthy and rats with MetS, and then depending on dietary patterns on six groups: healthy rats fed with Sd, healthy rats fed with Sd and SAE, rats with MetS fed with HFd, rats with MetS fed with HFd and SAE, rats with MetS fed with Sd, and rats with MetS fed with Sd and SAE. 4 weeks later, after an overnight fast (12–14 h), blood for determination of total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), index of lipid peroxidation (measured as TBARS), and FA was collected. Increased FA and lipid concentration found in MetS rats were reduced when changing dietary habits from HFd to Sd with or without SAE consumption. Consumption of SAE slightly affects the FA profiles, mostly palmitoleic acid in healthy rats and PUFA in MetS + HFd rats. Nevertheless, in a high-fat diet, SAE supplementation significantly decreases n-6/n-3 ratio, thereby decreasing systemic inflammation. Further researches are warranted to confirm these effects in humans.

Keywords

Aronia melanocarpa Fatty acids Lipid profiles Metabolic syndrome Dietary pattern 

Notes

Compliance with ethical standards

Conflicts of interest

The authors declare they have no conflicts of interest.

Ethical approval

This study was conducted in the laboratory for Cardiovascular Physiology (Faculty of Medical Sciences, University of Kragujevac, Serbia). Animals treatment and protocol employed were approved by the Ethical Committee for the welfare of experimental animals of the Faculty of Medical Sciences at the University of Kragujevac, Serbia on July 30, 2017 (number: 119-01-5/14/2017-09). The experimental procedures have been carried out in accordance with the EU Directive for the welfare of laboratory animals (86/609/EEC) and the principles of Good Laboratory Practice.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Petar Milic
    • 1
  • Jovana Jeremic
    • 2
  • Vladimir Zivkovic
    • 3
  • Ivan Srejovic
    • 3
  • Nevena Jeremic
    • 2
  • Jovana Bradic
    • 2
  • Tamara Nikolic Turnic
    • 2
  • Isidora Milosavljevic
    • 2
  • Sergey Bolevich
    • 4
  • Stefani Bolevich
    • 5
  • Milica Labudovic Borovic
    • 6
  • Aleksandra Arsic
    • 7
  • Miroslav Mitrovic
    • 8
  • Vladimir Jakovljevic
    • 3
    • 4
    Email author
  • Vesna Vucic
    • 7
  1. 1.High Medical School of Professional Studies in CuprijaCuprijaSerbia
  2. 2.Department of Pharmacy, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  3. 3.Department of Physiology, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  4. 4.Department of Human Pathology1st Moscow State Medical, University IM SechenovMoscowRussia
  5. 5.Department of Pathophysiology, 1st Moscow State MedicalUniversity IM SechenovMoscowRussia
  6. 6.Institute of Histology and Embryology “Aleksandar Dj. Kostic”, Faculty of MedicineUniversity of BelgradeBelgradeSerbia
  7. 7.Institute for Medical Research, Centre of Research Excellence in Nutrition and Metabolism, University of BelgradeBelgradeSerbia
  8. 8.PharmanovaBelgradeSerbia

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