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Molecular and Cellular Biochemistry

, Volume 453, Issue 1–2, pp 111–119 | Cite as

Stimulation role of epinephrine in transcription of the melatonin synthesis key enzyme AANAT in the pineal gland of broilers

  • Yongfen Wang
  • Zhen Zhang
  • Hongwei Guo
  • Pengying Li
  • Jingang LiEmail author
  • Lei Xi
  • Qiong Liu
Article
  • 143 Downloads

Abstract

Melatonin is a crucial neurohormone synthesized in the pineal gland that influences the physiology of animals. The molecular mechanism of norepinephrine control of the synthesis of melatonin is well documented; however, few reports have described the effects of epinephrine on the synthesis of melatonin. In this study, the effect of epinephrine on melatonin synthesis was investigated by adding different concentrations of epinephrine or norepinephrine to broiler pineal glands cultured in vitro. In addition, we investigated how epinephrine regulates the synthesis of melatonin and the transcription of the key melatonin synthesis enzyme AANAT. We determined the abundance of melatonin, norepinephrine, and epinephrine in broiler serum and the mRNA expression levels of key enzymes under different light conditions. The minimum concentrations of epinephrine and norepinephrine required to recover the melatonin synthesis rhythm in pineal cells were 10−13 and 10−11 mol/L, respectively. Under various light durations, epinephrine reached maximum levels two hours earlier than melatonin. These results demonstrate for the first time that epinephrine can increase the synthesis of melatonin by increasing the transcription of AANAT.

Keywords

Melatonin (MLT) Epinephrine (E) AANAT Pineal gland Broilers 

Notes

Acknowledgements

The Scientific and Technological Research Project in Henan Province (Grant Nos. 162102210297 and 172102110034), Major Scientific and Technological Project in Henan Province (Grant No. 141100110800), and Key Research Projects of Higher Education in Henan Province (Grant No. 17A230011) supported this work.

Author contributions

JL participated in conceiving and designing the study. YW performed most of the experiments, analyzed the data, and wrote the manuscript. ZZ, HG, PL, and QL participated in several of the experiments. All the authors discussed and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

11010_2018_3436_MOESM1_ESM.rar (814 kb)
Supplementary material 1 (RAR 813 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yongfen Wang
    • 1
    • 2
  • Zhen Zhang
    • 2
  • Hongwei Guo
    • 2
  • Pengying Li
    • 2
  • Jingang Li
    • 1
    Email author
  • Lei Xi
    • 2
  • Qiong Liu
    • 3
  1. 1.College of Life SciencesShaanxi Normal UniversityXi’anChina
  2. 2.College of Biology EngineeringHenan University of Animal Husbandry and EconomyZhengzhouChina
  3. 3.College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhouChina

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