Molecular and Cellular Biochemistry

, Volume 452, Issue 1–2, pp 83–94 | Cite as

Silibinin ameliorates amylin-induced pancreatic β-cell apoptosis partly via upregulation of GLP-1R/PKA pathway

  • Jing Yang
  • Yue Sun
  • Fanxing Xu
  • Weiwei Liu
  • Yingsi Mai
  • Toshihiko Hayashi
  • Shunji Hattori
  • Yuko Ushiki-Kaku
  • Satoshi Onodera
  • Shin-ichi Tashiro
  • Takashi IkejimaEmail author


The objective was to investigate the mechanism of the protective effect of silibinin on amylin/Aβ1−42-induced INS-1 cell apoptosis, with special reference to the roles of glucagon-like peptide-1 receptor (GLP-1R) and protein kinase A (PKA). The effects of silibinin on apoptosis, insulin secretion, GLP-1R, and PKA expression in the INS-1 cells treated with amylin/Aβ1−42 were examined. INS-1 cells exposed to amylin showed increased TUNEL-positive ratio, reduced expression of GLP-1R and PKA. GLP-1R antagonists or PKA inhibitor enhanced the expression of apoptosis-associated proteins and TUNEL-positive ratio. Silibinin exerted antiapoptotic effect on and upregulation of GLP-1R and PKA. However, Aβ1−42-induced INS-1 cell apoptosis, GLP-1R, and PKA expressions were not changed. Our results indicate that down-regulation of GLP-1R and PKA contributes to INS-1 cell apoptosis induced with amylin. Silibinin protects INS-1 cells from amylin-induced apoptosis through activation of GLP-1R/PKA signaling. Silibinin’s inhibition of the toxic effects of Aβ1−42 is independent of GLP-1R/PKA pathway.


Silibinin INS-1 cells GLP-1R PKA Apoptosis 



This study was supported by Innovation and Entrepreneurship Training Program of Shenyang Pharmaceutical University (201710163000006), Foundation of Liaoning Education Committee (51120427), and Doctoral Starting up Foundation of Liaoning Science and Technology Department (51120390).

Compliance with ethical standards

Conflict of interest

The authors have declared that there is no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jing Yang
    • 1
  • Yue Sun
    • 1
  • Fanxing Xu
    • 1
  • Weiwei Liu
    • 1
  • Yingsi Mai
    • 1
  • Toshihiko Hayashi
    • 1
    • 2
  • Shunji Hattori
    • 3
  • Yuko Ushiki-Kaku
    • 3
  • Satoshi Onodera
    • 4
  • Shin-ichi Tashiro
    • 5
  • Takashi Ikejima
    • 1
    Email author
  1. 1.Wuya College of InnovationShenyang Pharmaceutical UniversityShenyangPeople’s Republic of China
  2. 2.Department of Chemistry and Life Science, School of Advanced EngineeringKogakuin UniversityHachiojiJapan
  3. 3.Nippi Research Institute of BiomatrixTorideJapan
  4. 4.Department of Clinical and Biomedical SciencesShowa Pharmaceutical UniversityTokyoJapan
  5. 5.Department of Medical Education and Primary CareKyoto Prefectural University of MedicineKyotoJapan

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