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Molecular and Cellular Biochemistry

, Volume 450, Issue 1–2, pp 87–96 | Cite as

Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells

  • Qian Xu
  • Xiaoling Liu
  • Weiwei Liu
  • Toshihiko Hayashi
  • Masayuki Yamato
  • Hitomi Fujisaki
  • Shunji Hattori
  • Shin-ichi Tashiro
  • Satoshi Onodera
  • Takashi IkejimaEmail author
Article

Abstract

The extracellular matrix (ECM) is a major biomechanical environment for all cells in vivo, and tightly controls wound healing and cancer progression. Type I collagen (Col I) is the most abundant component in ECM and plays an essential role for cell motility control and migration beyond structural support. Our previous results showed that Col I increased the length of primary cilia and the expression of primary cilia-associated proteins in 3T3-L1 cells. The Hippo/YAP pathway serves as a major integrator of cell surface-mediated signals and regulates key processes for the development and maintenance of tissue functions. In this study, we investigated the role of Hippo/YAP signaling in primary cilia growth of cells cultured on Col I-coated plate, as well as the potential link between primary cilia and migration. At 2-day post-confluence, YAP localization in the nucleus was dramatically increased when the cells were cultured on Col I-coated plate, accompanied by cilia growth. YAP inhibitor verteporfin repressed the growth of primary cilia as well as the expressions of ciliogenesis-associated proteins in confluent 3T3-L1 cells cultured on Col I-coated plate. Moreover, knockdown of either YAP or IFT88, one of the ciliogenesis-associated proteins, reversed the migration of confluent 3T3-L1 cells promoted by Col I-coating. In conclusion, activation of YAP pathway by Col I-coating of culture plate for confluent 3T3-L1 cells is positively associated with the primary cilia growth, which eventually results in promoted migration.

Keywords

Collagen Primary cilia YAP Cell migration 3T3-L1 cells 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Qian Xu
    • 1
  • Xiaoling Liu
    • 1
  • Weiwei Liu
    • 1
  • Toshihiko Hayashi
    • 1
  • Masayuki Yamato
    • 2
  • Hitomi Fujisaki
    • 3
  • Shunji Hattori
    • 3
  • Shin-ichi Tashiro
    • 4
  • Satoshi Onodera
    • 5
  • Takashi Ikejima
    • 1
    Email author
  1. 1.China-Japan Research Institute of Medical and Pharmaceutical Sciences, Wuya Colleage of InnovationShenyang Pharmaceutical UniversityShenyangChina
  2. 2.Institute of Advanced Biomedical Engineering and SciencesTokyo Women’s Medical UniversityTokyoJapan
  3. 3.Nippi Research Institute of BiomatrixTorideJapan
  4. 4.Department of Medical Education & Primary CareKyoto Prefectural University of MedicineKyoto CityJapan
  5. 5.Department of Clinical and Pharmaceutical SciencesShowa Pharmaceutical UniversityTokyoJapan

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