Molecular and Cellular Biochemistry

, Volume 445, Issue 1–2, pp 169–178 | Cite as

Falcarindiol inhibits LPS-induced inflammation via attenuating MAPK and JAK-STAT signaling pathways in murine macrophage RAW 264.7 cells

  • Thamizhiniyan Venkatesan
  • Young-Woong Choi
  • Jennifer Lee
  • Young-Kyoon KimEmail author


Falcarindiol (FAD) is a natural polyacetylene compound found rich in many plants of the Umbelliferae family. Previously, we isolated FAD from the rhizome of Cnidium officinale Makino, which belongs to the Umbelliferae family and found it to have a significant inhibitory effect on lipopolysaccharide (LPS)-induced production of nitric oxide, a pro-inflammatory molecule in murine macrophage RAW 264.7 cells. In this study, we investigated its effect on the expression of other major pro-inflammatory molecules as well as the mechanism underlying these effects. Pre-treatment of RAW 264.7 cells with FAD suppressed LPS-stimulated mRNA expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) and thereby reduced the respective protein levels. Mechanistic studies demonstrated that FAD attenuated the LPS-induced activation of JNK, ERK, STAT1, and STAT3 signaling molecules. Moreover, we found that FAD did not influence LPS-induced activation of p38 and NFκB signaling pathways. Collectively, this study provides evidence that FAD inhibits the production of major pro-inflammatory molecules in LPS-challenged murine macrophages via suppression of JNK, ERK, and STAT signaling pathways.

Graphical Abstract


Cnidium officinale Rhizome Falcarindiol RAW 264.7 cells Anti-inflammatory activity 



Bicinchoninic acid


Bovine serum albumin


Complementary deoxyribonucleic acid


Dulbecco’s modified Eagle’s medium


Dimethyl sulfoxide

E. coli

Escherichia coli


Extracellular signal–regulated kinase




Fetal bovine serum




Hank’s balanced salt solution


4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)


Horseradish peroxidase


Inhibitory kappa B protein




Inducible nitric oxide synthase


Janus kinase-signal transducers and activators of transcription


c-Jun N-terminal kinase




Mitogen-activated protein kinases


Messenger RNA


(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)


Sodium chloride


Nuclear transcription factor kappa B


Nitric oxide


P38 Mitogen-activated protein kinase


Phosphate-buffered saline


Quantitative real-time polymerase chain reaction


Radioimmunoprecipitation assay


Ribonucleic acid


Sodium dodecyl sulfate


Signal transducers and activators of transcription factor 1


Signal transducers and activators of transcription factor 3


Toll-like receptor 4


Tumor necrosis factor α



The funding was provided by Forest Science & Technology Projects, Forest Service, Republic of Korea (Grant No. S211316L010110), Kookmin University (KR) research grant (Grant No. 2016)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Thamizhiniyan Venkatesan
    • 1
  • Young-Woong Choi
    • 1
  • Jennifer Lee
    • 1
  • Young-Kyoon Kim
    • 1
    Email author
  1. 1.Department of Forest Products and Biotechnology, College of Science and TechnologyKookmin UniversitySeoulSouth Korea

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