Molecular and Cellular Biochemistry

, Volume 441, Issue 1–2, pp 99–108 | Cite as

Knockdown of long non-coding RNA prostate cancer-associated ncRNA transcript 1 inhibits multidrug resistance and c-Myc-dependent aggressiveness in colorectal cancer Caco-2 and HT-29 cells

  • Lei Qiao
  • Xiangyu Liu
  • Yichao Tang
  • Zheng Zhao
  • Jilong Zhang
  • Huayan Liu
Article
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Abstract

The long non-coding RNA (lncRNA) prostate cancer-associated ncRNA transcript 1 (PCAT-1) has been shown to promote prostate cancer cell proliferation through c-Myc and is associated with the poor prognosis of CRC patients. In the current study, it was hypothesized that the effect of PCAT-1 on the aggressiveness of CRC cells was dependent on the function of c-Myc. Human CRC cell lines Caco-2 and HT-29 were transfected with specific PCAT-1 shRNAs, and cell migration, invasiveness, and resistance to 5-fluorouracil were measured. To elucidate the role of c-Myc in PCAT-1 function, c-Myc was overexpressed in PCAT-1-silenced CRC cells and the effect of c-Myc overexpression on the aggressiveness of PCAT-1-silenced cells was detected. The results showed that knockdown of PCAT-1 in CRC cells suppressed cell motility and invasiveness, and sensitized the cells to 5-fluorouracil, as evidenced by the reduced viability and induced apoptosis in PCAT-1-silenced cells compared to the parental cells in response to 5-fluorouracil treatment. The expression of c-Myc in PCAT-1-silenced CRC cells was down-regulated, and forced expression of c-Myc partially restored the invasiveness in PCAT-1-silenced cells. In summary, the findings outlined in the current study suggest that PCAT-1 regulates the invasiveness and drug resistance in CRC cells and that PCAT-1 may promote CRC cell invasion by modulating the expression of c-Myc.

Keywords

c-Myc Colorectal cancer Drug resistance Invasiveness PCAT-1 
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Notes

Acknowledgements

This study was supported by a grant from Science and Technology Plan Project of Shenyang City (No. F12-277-1-19).

Compliance with ethical standards

Conflict of interest

The authors disclose no conflict of interest.

Supplementary material

11010_2017_3177_MOESM1_ESM.doc (56 kb)
Supplementary material 1 (DOC 55 kb)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Lei Qiao
    • 1
  • Xiangyu Liu
    • 1
  • Yichao Tang
    • 1
  • Zheng Zhao
    • 1
  • Jilong Zhang
    • 1
  • Huayan Liu
    • 2
  1. 1.Department of Colorectal and Hernia Minimally Invasive SurgeryShengjing Hospital of China Medical UniversityShenyangPeople’s Republic of China
  2. 2.Department of NeurologyThe First Hospital of China Medical UniversityShenyangPeople’s Republic of China

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