Advertisement

Molecular and Cellular Biochemistry

, Volume 433, Issue 1–2, pp 169–176 | Cite as

Hyperoside inhibits the effects induced by oxidized low-density lipoprotein in vascular smooth muscle cells via oxLDL-LOX-1-ERK pathway

  • Zhengyu ZhangEmail author
  • Dongdong Zhang
  • Baoling Du
  • Zhiqiang Chen
Open Access
Article

Abstract

Hyperoside is a major active constituent in many medicinal plants traditionally used in Chinese medicines for their anti-inflammatory, antioxidative, and vascular protective effects. Recent studies have focused on the protective effects of hyperoside on hyperlipidemia. However, the molecular mechanisms underlying these effects are unknown. In this study, vascular smooth muscle cells (VSMCs) were treated in vitro with oxidized low-density lipoprotein (oxLDL) in the presence or absence of hyperoside. Western blotting, quantitative PCR, and tetrazolium assay were used to detect lectin-like oxLDL receptor-1 (LOX-1) expression and extracellular signal-regulated kinases (ERK) activation, and to determine VSMCs viability. The results demonstrated that oxLDL promoted LOX-1 expression, ERK activation, and proliferation in VSMCs. Hyperoside significantly inhibited the oxLDL-stimulated effects after long time exposure. However, it promoted ERK activation directly following a short incubation duration (25 min). In conclusion, hyperoside inhibits oxLDL-induced LOX-1 expression, ERK activation, and cell proliferation through the oxLDL-LOX-1-ERK pathway in VSMCs. Our findings suggest a novel role of hyperoside in treating and preventing atherosclerosis.

Keywords

Atherosclerosis Hyperoside oxLDL LOX-1 Receptor Cell signaling 

Introduction

Hyperoside is a flavonoid compound mainly found in herbal plants traditionally used in Chinese medicines and has many biological effects. Accumulating evidence suggests that hyperoside has neuroprotective, anti-cancer, anti-inflammatory, antioxidative, and vascular protective effects [1, 2, 3, 4, 5, 6]. However, the molecular mechanism underlying these effects is unknown.

Oxidation and inflammation both play important roles in the development of atherosclerosis and vascular remodeling. These effects are all involved in oxidized low-density lipoprotein (oxLDL)-induced vascular damage. In patients with hyperlipidemia, oxLDL can combine with scavenger receptors on the surface of vascular smooth muscle cells (VSMCs); lectin-like oxLDL receptor-1 (LOX-1) is one of the most important scavenger receptors on VSMCs. It is also well known that oxLDL promotes VSMC proliferation by activating extracellular signal-related kinases (ERK) [7, 8, 9]. Therefore, oxLDL-LOX-1-ERK pathway inhibition is important in the development of atherosclerosis and cardiovascular remodeling.

Several studies have reported the effect of hyperoside on LDL. Extracts from the leaves and flowers of Crataegus aronia that contain hyperoside decreased the serum total cholesterol and LDL of hypercholesterolemic rats [10]. In addition, hyperoside prolonged the lag time in conjugated diene formation in Cu2+-induced LDL in vitro [11]. These results indicate that flavonoids, including hyperoside, can not only decrease LDL levels but also inhibit LDL oxidation [10, 11, 12, 13, 14].

In the study of cellular signaling, we previously found that hyperoside attenuated ECV304 cell damage induced by advanced glycation end products [15]. Similarly, other studies reported that hyperoside protected cells by inhibiting oxidative stress, stimulating or inhibiting mitogen-activated protein kinase (MAPK) activation; these effects were direct or indirect [1, 2, 3, 6, 15, 16, 17]. For most of the indirect effects, modifying receptor expression is an important pathway. For example, hyperoside inhibited both VSMC proliferation in vitro and carotid artery ligation-induced neointimal formation in vivo by inducing Nur77 expression and activation [1]. Chu et al. reported that extract of Zanthoxylum ailanthoides that included rutin and hyperoside decreased the expression of scavenger receptor class AI (SR-AI) and CD36 [12]. However, there has been no report of the effect of hyperoside on the expression of LOX-1, an important receptor in oxLDL-induced atherosclerosis.

We believe that hyperoside has a protective effect against hyperlipidemia, but its molecular mechanism is largely unknown. Therefore, we carried out this study to elucidate whether hyperoside inhibits ERK activation and VSMC proliferation by downregulating LOX-1.

Materials and methods

VSMC culture

VSMCs were isolated by enzymatic digestion of the aortas of C57BL/6J mice using a modified version of a procedure described previously [18]. The isolated cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum, penicillin, and streptomycin at 37 °C in a humidified atmosphere of 5% CO2. The medium was changed every 2 days, and cells were passaged by treatment with 0.05% trypsin–0.02% EDTA solution. Experiments were conducted on VSMCs that had just achieved confluence. Cells between passages 5 and 15 were used; immunofluorescence staining and western blotting determined that the cells expressed smooth muscle α-actin.

All animal work has been conducted according to relevant national and international guidelines. Institutional Animal Care and Use Committee of Guangzhou Medical University has approved this research.

Western blotting

The western blotting procedures used were similar to that described previously with slight modifications [7]. Treated VSMCs were harvested in lysis buffer using protease inhibitors. The lysate suspension was centrifuged and protein concentration was assessed using a Bio-Rad protein assay. Heat-denatured proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrophoretically transferred onto nitrocellulose membranes. These were probed with antibodies against phosphorylated ERK (P-ERK; Cell Signaling Technology, Danvers, MA) and LOX-1 (Santa Cruz Biotechnology, Santa Cruz, CA) and re-probed with β-actin or pan-ERK antibody (Cell Signaling Technology, Danvers, MA). The bands were visualized using an enhanced chemiluminescence (ECL) detection system (GE Healthcare, Piscataway, NJ); intensity was quantitated using densitometry.

Real-time quantitative PCR (qPCR)

The qPCR was performed similarly to procedures described previously [15]. In brief, it was performed with Power SYBR Green PCR Master Mix (Takara Biotechnology, Dalian, China) on an ABI 7900 Real-Time PCR instrument according to the manufacturer’s instructions (Applied Biosystems, Foster City, CA). The primer sequences used were designed using Primer 3 (Whitehead Institute/MIT, Cambridge, MA) and confirmed by BLAST in GenBank. The forward (F) and reverse (R) qPCR primers for amplifying the LOX-1 [NM_001301096] and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) complementary DNA (cDNA) were synthesized by Takara Biotechnology as follows: GAPDH: F-5′-TGTGTCCGTCGTGGATCTGA-3′ and R-5′-TTGCTGTTGAAGTCGCAGGAG-3′; LOX1: F-5′-TGAAGCCTGCGAATGACGAG-3′ and R-5′-GTCACTGACAACACCAGGCAGAG-3′.

RNA interference

The procedures used for this experiment were similar to that described in our previous report [7]. The LOX-1 small interfering RNA (LOX-1-siRNA) target duplex sequences were synthesized by Invitrogen (Carlsbad, CA). A non-targeting siRNA duplex sequence (Stealth™ RNAi, Invitrogen, Carlsbad, CA) was used as the negative control (NC). VSMCs were transfected using 100 pmol of each siRNA duplex per well using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) and Opti-MEM (Invitrogen, Carlsbad, CA) according to the manufacturer’s recommendations. After 24-h transfection, the cells were serum-starved for an additional 24 h and then harvested to detect ERK phosphorylation by western blotting.

Tetrazolium (MTT) cell viability assay

Cell viability was determined using a CellTiter 96 Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, WI) according to the manufacturer’s instructions. Briefly, VSMCs were seeded in 96-well culture plates at 3000 cells/well. After incubation at 37 °C for 24 h, the medium was replaced with fresh medium supplemented with or without hyperoside (50 μg/ml) (Sigma Chemical Co., St. Louis, MO). After 10-min incubation, oxLDL (25 μg/ml) was added to the cells. Dye solution was added to each well 48 h after treatment and the cells were incubated at 37 °C for 2 h. Solubilization/stop solution was added to each well and the absorbance was measured at 570 nm using an enzyme-linked immunosorbent assay reader. Relative cell numbers were calculated after normalizing the absorbance to untreated cells. Cell viability was calculated relative to untreated cells.

Statistical analysis

Data from three independent experiments are expressed as the mean ± SD. Statistical analyses were performed with one-way analysis of variance (ANOVA). P-values below 0.05 were considered significant.

Results

Hyperoside downregulates oxLDL-induced LOX-1 expression in VSMCs

To determine whether hyperoside could affect LOX-1 expression induced by oxLDL, quiescent VSMCs were treated with oxLDL with or without hyperoside. Real-time qPCR (Fig. 1a) showed that with the concentration of 50 and 100 μg/ml, hyperoside significantly inhibited oxLDL-induced LOX-1 upregulation. Then hyperoside (50 μg/ml) was used in western blotting (Fig. 1b, c). The results showed that in the absence of hyperoside, oxLDL significantly increased LOX-1 expression relative to the NC (P < 0.05). In contrast, hyperoside significantly inhibited this effect (P < 0.05). These results suggest that oxLDL may induce LOX-1 upregulation and that hyperoside inhibits this effect.
Fig. 1

Hyperoside downregulates oxLDL-induced LOX-1 expression in VSMCs. a qPCR shows LOX-1 expression levels in VSMCs treated with oxLDL in different concentrations of hyperoside for 12 h. b Western blot shows LOX-1 expression levels in VSMCs treated with oxLDL in the absence or presence of hyperoside for 24 h. c The statistical results of (b) are (a) versus NC (P < 0.01), (b) versus NC (P < 0.05), and (c) versus oxLDL (P < 0.05); results are from three independent experiments. NC negative control, H hyperoside

OxLDL promotes cell viability and hyperoside inhibits these effects

OxLDL promotes VSMC proliferation [19, 20]. In this study, we detected the effect of hyperoside on oxLDL-stimulated cells by MTT assay. The cell viability between the oxLDL-treated and NC groups was significantly different (P < 0.05), indicating that oxLDL promoted cell viability in VSMCs. The viability of cells treated with oxLDL and hyperoside was significantly lower relative to cells treated with oxLDL alone (P < 0.05; Fig. 2), indicating that hyperoside inhibits oxLDL-induced cell viability. We also found that hyperoside alone inhibited cell viability compared to the NC.
Fig. 2

Hyperoside inhibits cell viability and attenuates the effects of oxLDL. Cells were pretreated with or without hyperoside (50 μg/ml). After 10-min incubation, oxLDL (25 μg/ml) was added to the cells. Dye solution was added to each well 48 h after treatment and the cells were incubated at 37 °C for 2 h. Results shown are a percentage of the NC, (a) versus NC (P < 0.05) and (b) versus oxLDL (P < 0.05). NC negative control, H hyperoside

Hyperoside cannot directly inhibit oxLDL-induced ERK activation

To date, no data are available on the effects of hyperoside in oxLDL-induced ERK activation in VSMCs. P-ERK is activated ERK; more P-ERK can be detected when certain stimuli activate ERK. Western blotting (Fig. 3) showed that oxLDL induced ERK activation. No significant alteration was detected when VSMCs were incubated with oxLDL and hyperoside (P > 0.05). Compared to the NC, hyperoside significantly promoted ERK activation and reached peak after 25-min incubation. These results suggest that hyperoside may not have a direct inhibitory effect on oxLDL-induced ERK activation.
Fig. 3

Hyperoside does not inhibit oxLDL-induced ERK activation in VSMCs directly. a Cells were treated with hyperoside from 5 min to 1 h; b cells were pretreated with hyperoside and then treated with oxLDL. Western blot shows P-ERK activation. b, d the statistical results of (a, c) are (a) versus NC (P < 0.05), (b) versus NC (P > 0.05) and (c) versus oxLDL (P > 0.05). Results are from three independent experiments. NC negative control, H hyperoside

Hyperoside inhibits oxLDL-induced ERK activation by downregulating VSMC LOX-1 expression

If hyperoside could not inhibit ERK activation, how could it inhibit oxLDL-induced cell viability? To elucidate the effect of hyperoside on the oxLDL-LOX-1-ERK signaling pathway, cells were treated with or without hyperoside for 24 h, and then treated with oxLDL for 15 min before harvesting for western blotting to detect ERK activation. Western blotting (Fig. 4a, b) showed significantly decreased ERK activation in VSMCs pretreated with hyperoside compared with oxLDL group (P < 0.05). Compared with NC group, no significant alteration was detected when VSMCs were incubated with hyperoside for 24 h. These results suggested that hyperoside may inhibit oxLDL-induced ERK activation by altering the expression of oxLDL receptors. To confirm this hypothesis, cells were pretreated with LOX-1-siRNA or hyperoside for 48 h, and then treated with oxLDL for 15 min before harvesting. Western blotting (Fig. 4c, d) showed that ERK activation in cells pretreated with LOX-1-siRNA and hyperoside was inhibited. No differences were detected in the groups not treated with oxLDL. These results indicate that hyperoside has the similar function as LOX-1-siRNA in inhibiting the expression of LOX-1.
Fig. 4

Hyperoside inhibits oxLDL-induced ERK activation by downregulating LOX-1 expression in VSMCs. a VSMCs were pretreated with or without hyperoside and then treated with oxLDL. Western blot shows the ERK activation. c Cells were pretreated with LOX-1-siRNA or hyperoside, serum-starved, and then treated with oxLDL. Western blotting detected ERK activation. b, d The statistical results of (a, c) are (a) versus NC (P < 0.05) and (b) versus oxLDL (P < 0.05). Results are from three independent experiments; LIPO Lipofectamine 2000, H hyperoside

Discussion

This study demonstrated that hyperoside has protective effects against oxLDL-induced LOX-1 expression, ERK activation, and proliferation in VSMCs. First, hyperoside suppresses LOX-1 expression at gene and protein level. Second, it inhibits VSMC viability. Third, it directly promotes ERK activation following short-duration incubation (25 min), but suppresses oxLDL-induced ERK activation by downregulating LOX-1 expression, and eventually suppresses cell proliferation.

Hyperoside has anti-inflammatory, antioxidative, and vascular protective effects [1, 21, 22, 23]. Accordingly, we detected its effect on oxLDL-induced LOX-1 expression. As expected, hyperoside suppressed LOX-1 expression at gene and protein level (Fig. 1). Next, we detected cell viability by incubating VSMCs with hyperoside in the absence or presence of oxLDL, and found that hyperoside inhibits VSMC viability (Fig. 2). This result is similar to that of another report [1]. However, the signaling pathway of these effects is unknown.

Recent findings have demonstrated that hyperoside promotes ERK activation in a time- and dose-dependent manner. ERK is the downstream signaling molecule of oxLDL-LOX-1 [7, 24]. In this study, 25-min treatment with hyperoside promoted ERK activation. However, there were no significant differences in ERK activation in cells treated with hyperoside and oxLDL together as compared to oxLDL-treated cells. The ERK activation of the two groups was significantly higher than that of the NC (Fig. 3). These results indicate that hyperoside promotes ERK activation alone and cannot inhibit oxLDL-induced ERK activation. We also did not find a synergistic effect between hyperoside- and oxLDL-induced ERK activation (Fig. 3). However, this was a short-duration study. When cells were pretreated with hyperoside for 24 h and then incubated with oxLDL for 15 min, we obtained wholly different results in that hyperoside pre-treatment inhibited oxLDL-induced ERK activation (Fig. 4a, b).

The short-duration promoting effect of hyperoside on ERK activation should promote cell proliferation or viability, while our study indicated that hyperoside suppresses oxLDL-induced promotion of cell viability (Fig. 2). There are two possible reasons for this: one is that hyperoside triggered other signaling pathways that induce apoptosis. Some have reported the effect of hyperoside on P38 [16, 25]. The other possible reason is that hyperoside alters the expression of some receptors on the oxLDL-related signaling pathway. In our study, we found that hyperoside suppressed the expression of LOX-1, one of the most important receptors on the oxLDL signaling pathway. Therefore, lower LOX-1 expression decreased formation of the oxLDL–LOX-1 complex, attenuating the effects of oxLDL and suppressing ERK activation and cell viability (Figs. 2, 4a, b). Other studies have also published similar reports in that hyperoside inhibited the expression of Nur77, SR-AI, and CD36 [1, 12].

To further confirm our findings, cells were incubated with LOX-1-siRNA or hyperoside, and then treated with oxLDL. Previously, we reported that siRNA inhibits ERK activation [7]. In the present study, hyperoside inhibited ERK activation, a similar effect to that of siRNA (Fig. 4c, d). These results indicate that hyperoside at least partially inhibits the oxLDL-LOX-1-ERK signaling pathway by suppressing LOX-1 expression.

In conclusion, we illustrate a novel role of hyperoside in inhibiting oxLDL-induced ERK activation and cell proliferation in VSMCs through the oxLDL-LOX-1-ERK pathway (Fig. 5). These findings might advance current understanding of the effects of hyperoside on vascular protection.
Fig. 5

Diagram shows the effect of hyperoside on the oxLDL-LOX-1-ERK signaling pathway and VSMC proliferation. Increased blood LDL can trigger oxLDL increases in the walls of vein grafts and arteries. As a LOX-1 ligand, oxLDL can activate LOX-1 and its downstream signaling molecules, including ERK. Activated ERK leads to LOX-1 overexpression and promotes VSMCs viability, thereby altering vascular structure and function. Blocking LOX-1 and its downstream signaling molecules by hyperoside might inhibit vascular remodeling induced by oxLDL

Notes

Acknowledgements

This work was supported by grants from the Natural Science Foundation of Guangdong Province, China (No. 2014A030310207), Guangzhou Medical University Foundation (No. 2013c13), Foundation of Guangdong Provincial Bureau of traditional Chinese Medicine (No. 20171181), Foundation of Guangdong Provincial Planning Office of Philosophy and Social Science (No. GD16XWW04) and Innovation Project of Institutions of Higher Learning in Guangdong Province (No. 2016GXJK076).

References

  1. 1.
    Huo Y, Yi B, Chen M, Wang N, Chen P, Guo C, Sun J (2014) Induction of Nur77 by hyperoside inhibits vascular smooth muscle cell proliferation and neointimal formation. Biochem Pharmacol 92:590–598. doi: 10.1016/j.bcp.2014.09.021 CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Ku SK, Zhou W, Lee W, Han MS, Na M, Bae JS (2015) Anti-inflammatory effects of hyperoside in human endothelial cells and in mice. Inflammation 38:784–799. doi: 10.1007/s10753-014-9989-8 CrossRefPubMedGoogle Scholar
  3. 3.
    Park JY, Han X, Piao MJ, Oh MC, Fernando PM, Kang KA, Ryu YS, Jung U, Kim IG, Hyun JW (2016) Hyperoside induces endogenous antioxidant system to alleviate oxidative stress. J Cancer Prev 21:41–47. doi: 10.15430/JCP.2016.21.1.41 CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Fu T, Wang L, Jin XN, Sui HJ, Liu Z, Jin Y (2016) Hyperoside induces both autophagy and apoptosis in non-small cell lung cancer cells in vitro. Acta Pharmacol Sin 37:505–518. doi: 10.1038/aps.2015.148 CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Jin XN, Yan EZ, Wang HM, Sui HJ, Liu Z, Gao W, Jin Y (2016) Hyperoside exerts anti-inflammatory and anti-arthritic effects in LPS-stimulated human fibroblast-like synoviocytes in vitro and in mice with collagen-induced arthritis. Acta Pharmacol Sin. doi: 10.1038/aps.2016.7 Google Scholar
  6. 6.
    Liu RL, Xiong QJ, Shu Q, Wu WN, Cheng J, Fu H, Wang F, Chen JG, Hu ZL (2012) Hyperoside protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via nitric oxide signal pathway. Brain Res 1469:164–173. doi: 10.1016/j.brainres.2012.06.044 CrossRefPubMedGoogle Scholar
  7. 7.
    Zhang Z, Zhang M, Li Y, Liu S, Ping S, Wang J, Ning F, Xie F, Li C (2013) Simvastatin inhibits the additive activation of ERK1/2 and proliferation of rat vascular smooth muscle cells induced by combined mechanical stress and oxLDL through LOX-1 pathway. Cell Signal 25:332–340. doi: 10.1016/j.cellsig.2012.10.006 CrossRefPubMedGoogle Scholar
  8. 8.
    Maziere C, Gomila C, Maziere JC (2010) Oxidized low-density lipoprotein increases osteopontin expression by generation of oxidative stress. Free Radic Biol Med 48:1382–1387. doi: 10.1016/j.freeradbiomed.2010.02.022 CrossRefPubMedGoogle Scholar
  9. 9.
    Maziere C, Trecherel E, Ausseil J, Louandre C, Maziere JC (2011) Oxidized low density lipoprotein induces cyclin. A synthesis. involvement of ERK, JNK and NFkappaB. Atherosclerosis 218:308–313. doi: 10.1016/j.atherosclerosis.2011.06.034 CrossRefPubMedGoogle Scholar
  10. 10.
    Al-Hallaq EK, Afifi FU, Abdalla SS (2012) Evaluation of the hypocholesterolemic effect and phytochemical screening of the hydroethanolic extract of Crataegus aronia from Jordan. Nat Prod Commun 7:35–38PubMedGoogle Scholar
  11. 11.
    Kim DW, Yokozawa T, Hattori M, Kadota S, Namba T (2000) Inhibitory effects of an aqueous extract of Apocynum venetum leaves and its constituents on Cu(2+)-induced oxidative modification of low density lipoprotein. Phytother Res 14:501–504CrossRefPubMedGoogle Scholar
  12. 12.
    Chu CY, Lee HJ, Chu CY, Yin YF, Tseng TH (2009) Protective effects of leaf extract of Zanthoxylum ailanthoides on oxidation of low-density lipoprotein and accumulation of lipid in differentiated THP-1 cells. Food Chem Toxicol 47:1265–1271. doi: 10.1016/j.fct.2009.02.020 CrossRefPubMedGoogle Scholar
  13. 13.
    Quettier-Deleu C, Voiselle G, Fruchart JC, Duriez P, Teissier E, Bailleul F, Vasseur J, Trotin F (2003) Hawthorn extracts inhibit LDL oxidation. Pharmazie 58:577–581PubMedGoogle Scholar
  14. 14.
    Vaughn K, McClain C, Carrier DJ, Wallace S, King J, Nagarajan S, Clausen E (2007) Effect of Albizia julibrissin water extracts on low-density lipoprotein oxidization. J Agric Food Chem 55:4704–4709. doi: 10.1021/jf063458e CrossRefPubMedGoogle Scholar
  15. 15.
    Zhang Z, Sethiel MS, Shen W, Liao S, Zou Y (2013) Hyperoside downregulates the receptor for advanced glycation end products (RAGE) and promotes proliferation in ECV304 cells via the c-Jun N-terminal kinases (JNK) pathway following stimulation by advanced glycation end-products in vitro. Int J Mol Sci 14:22697–22707. doi: 10.3390/ijms141122697 CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Xing HY, Liu Y, Chen JH, Sun FJ, Shi HQ, Xia PY (2011) Hyperoside attenuates hydrogen peroxide-induced L02 cell damage via MAPK-dependent Keap(1)-Nrf(2)-ARE signaling pathway. Biochem Biophys Res Commun 410:759–765. doi: 10.1016/j.bbrc.2011.06.046 CrossRefPubMedGoogle Scholar
  17. 17.
    Li ZL, Liu JC, Hu J, Li XQ, Wang SW, Yi DH, Zhao MG (2012) Protective effects of hyperoside against human umbilical vein endothelial cell damage induced by hydrogen peroxide. J Ethnopharmacol 139:388–394. doi: 10.1016/j.jep.2011.11.020 CrossRefPubMedGoogle Scholar
  18. 18.
    Liu S, Li Y, Zhang Z, Xie F, Xu Q, Huang X, Huang J, Li C (2013) Alpha1-adrenergic receptors mediate combined signals initiated by mechanical stretch stress and norepinephrine leading to accelerated mouse vein graft atherosclerosis. J Vasc Surg 57:1645–1656. doi: 10.1016/j.jvs.2012.09.061 CrossRefPubMedGoogle Scholar
  19. 19.
    Kiyan Y, Tkachuk S, Hilfiker-Kleiner D, Haller H, Fuhrman B, Dumler I (2014) oxLDL induces inflammatory responses in vascular smooth muscle cells via urokinase receptor association with CD36 and TLR4. J Mol Cell Cardiol 66:72–82. doi: 10.1016/j.yjmcc.2013.11.005 CrossRefPubMedGoogle Scholar
  20. 20.
    Liu J, Ren Y, Kang L, Zhang L (2014) Oxidized low-density lipoprotein increases the proliferation and migration of human coronary artery smooth muscle cells through the upregulation of osteopontin. Int J Mol Med 33:1341–1347. doi: 10.3892/ijmm.2014.1681 PubMedGoogle Scholar
  21. 21.
    Kim SJ, Um JY, Lee JY (2011) Anti-inflammatory activity of hyperoside through the suppression of nuclear factor-kappaB activation in mouse peritoneal macrophages. Am J Chin Med 39:171–181. doi: 10.1142/S0192415X11008737 CrossRefPubMedGoogle Scholar
  22. 22.
    Choi JH, Kim DW, Yun N, Choi JS, Islam MN, Kim YS, Lee SM (2011) Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice. J Nat Prod 74:1055–1060. doi: 10.1021/np200001x CrossRefPubMedGoogle Scholar
  23. 23.
    Kupeli Akkol E, Bahadir Acikara O, Suntar I, Ergene B, Saltan Citoglu G (2012) Ethnopharmacological evaluation of some Scorzonera species: in vivo anti-inflammatory and antinociceptive effects. J Ethnopharmacol 140:261–270. doi: 10.1016/j.jep.2012.01.015 CrossRefPubMedGoogle Scholar
  24. 24.
    Makino J, Asai R, Hashimoto M, Kamiya T, Hara H, Ninomiya M, Koketsu M, Adachi T (2016) Suppression of EC-SOD by oxLDL during vascular smooth muscle cell proliferation. J Cell Biochem. doi: 10.1002/jcb.25542 PubMedGoogle Scholar
  25. 25.
    Kim YS, Jung DH, Lee IS, Pyun BJ, Kim JS (2016) Osteomeles schwerinae extracts inhibits the binding to receptors of advanced glycation end products and TGF-beta1 expression in mesangial cells under diabetic conditions. Phytomedicine 23:388–397. doi: 10.1016/j.phymed.2016.02.005 CrossRefPubMedGoogle Scholar

Copyright information

© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Zhengyu Zhang
    • 1
    • 2
    Email author
  • Dongdong Zhang
    • 3
  • Baoling Du
    • 1
    • 2
  • Zhiqiang Chen
    • 4
  1. 1.Guangzhou Institute of Cardiovascular DiseaseThe Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhouChina
  2. 2.Department of CardiologyGuangzhou Medical UniversityGuangzhouChina
  3. 3.Department of UltrasonicLiaocheng People’s HospitalLiaochengChina
  4. 4.Department of OrthopedicsLiaocheng People’s HospitalLiaochengChina

Personalised recommendations