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Molecular and Cellular Biochemistry

, Volume 430, Issue 1–2, pp 47–56 | Cite as

A novel antisense long non-coding RNA SATB2-AS1 overexpresses in osteosarcoma and increases cell proliferation and growth

  • Si-Hong Liu
  • Jian-Wei Zhu
  • Hui-Hua Xu
  • Gui-Qiang Zhang
  • Yan Wang
  • Ya-Min Liu
  • Jun-Bo Liang
  • Yu-Xuan Wang
  • Yong WuEmail author
  • Qi-Feng GuoEmail author
Article

Abstract

The knowledge regarding the importance of long non-coding RNAs (lncRNAs), a new class of genes, is very sparse in osteosarcoma. In the present study, we describe the expression profile of lncRNAs in osteosarcomas compared with paired adjacent non-cancerous tissue (n = 7) using microarray analysis. A total of 25,733 lncRNAs were identified in osteosarcoma; 1995 lncRNAs were consistently upregulated and 2226 lncRNAs were consistently under-regulated in all samples analyzed (≥2.0-fold, p < 0.05). We have validated three over-regulated and three under-regulated lncRNAs in patient samples (n = 7). The antisense transcript of SATB2 protein (SATB2-AS1) was identified as one of the upregulated lncRNAs. The SATB2-AS1 is a 3197-bp lncRNA on chromosome 2. This is the first report, where we have documented the increased expression of SATB2-AS1 in osteosarcoma patients and in human osteosarcoma cancer cell lines (U2OS, HOS, MG63). SATB2-AS1 expression was significantly higher in the metastatic tumors compared to non-metastatic tumors. In vitro gain and loss of function approaches demonstrated that SATB2-AS1 regulates cell cycle, cell proliferation, and cell growth. In addition, SATB2-AS1 affects the translational expression of SATB2 gene. Our data demonstrate that an antisense non-coding RNA regulates the expression of its sense gene, and increases the cell growth, therefore pointing the pivotal functions of SATB2-AS1 in osteosarcoma.

Keywords

LncRNA Osteosarcoma Antisense Cell proliferation Metastasis Cell Cycle 

Notes

Acknowledgements

The National Nature Science Foundation of China (Nos. 31601031, 81272941, 81201864), and The Guangdong Planned Project of Science and Technology (2014A020212009) financially supported the present study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

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11010_2017_2953_MOESM3_ESM.docx (39 kb)
Supplementary material 3 (DOCX 39 KB)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Si-Hong Liu
    • 1
    • 2
  • Jian-Wei Zhu
    • 2
  • Hui-Hua Xu
    • 2
  • Gui-Qiang Zhang
    • 2
  • Yan Wang
    • 2
  • Ya-Min Liu
    • 2
  • Jun-Bo Liang
    • 2
  • Yu-Xuan Wang
    • 2
  • Yong Wu
    • 3
    Email author
  • Qi-Feng Guo
    • 2
    Email author
  1. 1.Jinan UniversityGuangzhou,People’s Republic of China
  2. 2.Department of Orthopaedics, Guangzhou First People’s HospitalGuangzhou Medical UniversityGuangzhou,People’s Republic of China
  3. 3.Department of Oncology, Guangzhou First People’s HospitalGuangzhou Medical UniversityGuangzhouPeople’s Republic of China

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