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RETRACTED ARTICLE: MicroRNA-20b suppresses the expression of ZFP-148 in viral myocarditis

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This article was retracted on 04 November 2022

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Abstract

Viral myocarditis is a common cardiovascular disease, which seriously endangers the health of people and even leads to sudden unexpected death. MicroRNAs play very important roles in various physical and pathological processes including cardiogenesis and heart diseases. In recent years, miR-20b has been implicated in various diseases such as breast cancer, gastric cancer, hepatocellular carcinoma, cardiovascular diseases. However, the function of miR-20b in the pathological progress of viral myocarditis has not been reported. In this study, we found that miR-20b was up-regulated in mouse heart tissues post Coxsackievirus B3 (CVB3) infection. Bioinformatics analysis identified ZFP-148, a transcription factor that plays essential roles in the regulation of virus replication, is one of the predicted targets of miR-20b. MiR-20b expression was found to be up-regulated and ZFP-148 protein level was markedly repressed during viral myocarditis. Further studies demonstrated that miR-20b directly binds to the 3′-UTR of ZFP-148 and suppresses its translation. Moreover, aberrant expression of miR-20b promoted the expression of anti-apoptosis proteins Bcl-2 and Bcl-xL, suggesting that altered gene expression might promote cardiomyocytes survival in viral myocarditis. Our findings indicated that miR-20b might be a potential therapeutic target for CVB3-induced viral myocarditis and a useful marker for the diagnosis of viral myocarditis.

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Acknowledgements

We express gratitude for the excellent technical assistance and laboratory equipments provided by Soochow University. Meanwhile, we are grateful for the support of the Institute of cardiovascular diseases at Fudan University. This study was supported by the National Science Foundation of China (Nos. 81302617 and 571848), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Author Contributions

X.H.F., L.J.Y., and Z.S.H. conception and design of research; X.H.F., G.X.T., L.J.Y., X.X.H., D.Y.J., and Z.S.H. performed experiments; X.H.F., G.X.T., X.X.H., D.Y.J., and Z.S.H. analyzed data; X.H.F., G.X.T., L.J.Y., D.Y.J., and Z.S.H. interpreted results of experiments; X.H.F. prepared figures; X.H.F. and G.X.T. drafted manuscript; X.H.F., G.X.T., L.J.Y., X.X.H., D.Y.J., and Z.S.H. edited and revised manuscript; X.H.F., G.X.T., L.J.Y., X.X.H., D.Y.J., and Z.S.H. approved final version of manuscript.

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    Authors and Affiliations

    1. Department of Forensic Medicine, Soochow University, Suzhou Dushuhu High Educational Town, Suzhou, Jiangsu, 215123, People’s Republic of China

      Hong-Fei Xu, Xiang-Ting Gao, Xue-Hua Xu, Jun Hu & Shao-Hua Zhu

    2. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Xuhui, Shanghai, 200032, People’s Republic of China

      Jun-Yi Lin

    3. Department of dermatological, The second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215123, People’s Republic of China

      Yu-Jie Ding

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    Hong-Fei Xu, Xiang-Ting Gao and Jun-Yi Lin have contribute equally to this work and they share first authorship.

    Hong-Fei Xu, Yu-Jie Ding, and Shao-Hua Zhu have contribute equally to this work and they share corresponding authorship.

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    Xu, HF., Gao, XT., Lin, JY. et al. RETRACTED ARTICLE: MicroRNA-20b suppresses the expression of ZFP-148 in viral myocarditis. Mol Cell Biochem 429, 199–210 (2017). https://doi.org/10.1007/s11010-017-2947-7

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