Lymphocyte genotoxicity and protective effect of Calyptranthes tricona (Myrtaceae) against H2O2-induced cell death in MCF-7 cells
- 356 Downloads
Calyptranthes tricona is a species (Myrtaceae) native to South Brazil. Plants belonging to this family are folkloric used for analgesia, inflammation, and infectious diseases. However, little is known about the toxic potential of C. tricona. The present study aimed to evaluate the antioxidant activity of C. tricona ethanol and hexane leaf extracts, as well as verify their effect on human lymphocytes and MCF-7 cells. The extracts were subjected to preliminary phytochemical screening, antioxidant activity using DPPH and ORAC methods. Genotoxic and mutagenic effects in cultured human lymphocytes were assessed using the comet assay and the micronucleus assay, respectively. In addition, cell viability by MTT assay and fluorometric analysis of mitochondrial potential and caspases-9 activity were performed in order to verify the possible effects of both extracts on H2O2-induced cell death of MCF-7 cells. Our findings revealed that the phenol content and the antioxidant activity were only present in the ethanol extract. Also, the phytochemical screening presented steroids, triterpenoids, condensed tannins, and flavones as the main compounds. However, both extracts were capable of inducing concentration-dependent DNA damage in human lymphocytes. When treating MCF-7 cells with the extracts, both of them inhibited MCF-7 cell death in response to oxidative stress through a decrease of mitochondrial depolarization and caspases-9 activity. Thus, our results need to be considered in future in vitro and in vivo studies of C. tricona effects. In the meanwhile, we recommend caution in the acute/chronic use of this homemade preparation for medicinal purpose.
KeywordsCalyptranthes tricona Genotoxicity Human lymphocytes, MCF-7, cytoprotection
We thank Ms. Andréa Horst from the Physiology Department of Universidade Federal do Rio Grande do Sul (UFRGS) and Dr. Adriane Pozzobon from Centro Universitário UNIVATES for technical assistance. This work was supported by Centro Universitário UNIVATES and the National Counsel of Technological and Scientific Development (CNPq). DMK, DF, and SB are the recipients of Master grants and a postdoctoral grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes), respectively.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 5.de Figueiroa EO, da Silva LCN, de Melo CM, Neves JK, da Silva NH, Pereira VR, Correia MT (2013) Evaluation of antioxidant, immunomodulatory, and cytotoxic action of fractions from Eugenia uniflora L. and Eugenia malaccensis L.: correlation with polyphenol and flavanoid content. Sci World J. doi: 10.1155/2013/125027 Google Scholar
- 9.Sagrillo MR, Garcia LF, de Souza Filho OC, Duarte MM, Ribeiro EE, Cadona FC, da Cruz IB (2015) Tucuma fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes. Food Chem 173:741–748. doi: 10.1016/j.foodchem.2014.10.067 CrossRefPubMedGoogle Scholar
- 11.da Silva Brum E, da Rosa Moreira L, da Silva AR, Boligon AA, Carvalho FB, Athayde ML, Brandao R, Oliveira SM (2016) Tabernaemontana catharinensis ethyl acetate fraction presents antinociceptive activity without causing toxicological effects in mice. J Ethnopharmacol. doi: 10.1016/j.jep.2016.06.036 Google Scholar
- 18.RCoreTeam (2014) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
- 29.Wang WH, Tyan YC, Chen ZS, Lin CG, Yang MH, Yuan SS, Tsai WC (2014) Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells. Biomed Res Int 2014:185946. doi: 10.1155/2014/185946 PubMedPubMedCentralGoogle Scholar
- 32.de Souza Filho OC, Sagrillo MR, Garcia LF, Machado AK, Cadona F, Ribeiro EE, Duarte MM, Morel AF, da Cruz IB (2013) The in vitro genotoxic effect of Tucuma (Astrocaryum aculeatum), an Amazonian fruit rich in carotenoids. J Med Food 16:1013–1021. doi: 10.1089/jmf.2012.0287 CrossRefPubMedGoogle Scholar
- 35.Tor YS, Yazan LS, Foo JB, Wibowo A, Ismail N, Cheah YK, Abdullah R, Ismail M, Ismail IS, Yeap SK (2015) Induction of apoptosis in MCF-7 cells via oxidative stress generation, mitochondria-dependent and caspase-independent pathway by ethyl acetate extract of Dillenia suffruticosa and its chemical profile. PLoS One. doi: 10.1371/journal.pone.0127441 PubMedPubMedCentralGoogle Scholar