Molecular and Cellular Biochemistry

, Volume 409, Issue 1–2, pp 51–58 | Cite as

Edaravone protects osteoblastic cells from dexamethasone through inhibiting oxidative stress and mPTP opening

  • Wen-xiao Sun
  • Hai-ya Zheng
  • Jun LanEmail author


Existing evidences have emphasized an important role of oxidative stress in dexamethasone (Dex)-induced osteoblastic cell damages. Here, we investigated the possible anti-Dex activity of edaravone in osteoblastic cells, and studied the underlying mechanisms. We showed that edaravone dose-dependently attenuated Dex-induced death and apoptosis of established human or murine osteoblastic cells. Further, Dex-mediated damages to primary murine osteoblasts were also alleviated by edaravone. In osteoblastic cells/osteoblasts, Dex induced significant oxidative stresses, tested by increased levels of reactive oxygen species and lipid peroxidation, which were remarkably inhibited by edaravone. Meanwhile, edaravone repressed Dex-induced mitochondrial permeability transition pore (mPTP) opening, or mitochondrial membrane potential reduction, in osteoblastic cells/osteoblasts. Significantly, edaravone-induced osteoblast-protective activity against Dex was alleviated with mPTP inhibition through cyclosporin A or cyclophilin-D siRNA. Together, we demonstrate that edaravone protects osteoblasts from Dex-induced damages probably through inhibiting oxidative stresses and following mPTP opening.


Dexamethasone Osteoblasts Edaravone Oxidative stress Osteonecrosis 



This study is supported by the National Natural Science Foundation of China.

Compliance with Ethical Standards

Conflict of interest

No conflict of interests were stated.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Orthopedic and Hand Surgery, Liyuan HospitalHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Clinical LaboratoryThe People’s Hospital of LishuiLishuiChina
  3. 3.Department of OrthopaedicsThe People’s Hospital of LishuiLishuiChina

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