Molecular and Cellular Biochemistry

, Volume 399, Issue 1–2, pp 123–130 | Cite as

MicroRNA-126 attenuates palmitate-induced apoptosis by targeting TRAF7 in HUVECs

Article

Abstract

The aim of the present study was to explore the role of miR-126 in palmitate-induced HUVECs apoptosis and the possible mechanisms. Palmitate inhibited miR-126 expression in HUVECs, increased reactive oxygen species (ROS) production, and induced apoptosis as determined by up-regulation of caspase-3 activity and DNA fragmentation. Overexpression of miR-126 decreased ROS production, TNF-α expression, and apoptosis in palmitate-stimulated HUVECs. In contrast, miR-126 antagomir enhanced palmitate-induced ROS production, TNF-α expression, and apoptosis. The induction of miR-126 correlated with a reduction in TRAF7. We further showed that miR-126 targeted and inhibited TRAF7 expression through target sites located in the 3′ untranslated region of TRAF7 mRNA. In concordance, miR-126 mimic reduced TRAF7 protein in HUVECs, whereas the inhibition of miR-126 increased it. This study demonstrates an anti-apoptotic role of miR-126 in HUVECs and identifies TRAF7 as a direct target of miR-126 in HUVECs.

Keywords

miR-126 Palmitate ROS production TRAF7 Apoptosis 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81070232, 81270372, 81470568), Key Project of Chinese Ministry of Education (No. 212077), Grants for Scientific Research of BSKY (No. XJ201107, XJ2008015) from Anhui Medical University.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Yi Wang
    • 1
    • 2
    • 3
  • Feng Wang
    • 2
  • Yan Wu
    • 2
    • 3
  • Li Zuo
    • 2
    • 3
  • Sumei Zhang
    • 2
    • 3
  • Qing Zhou
    • 2
  • Wei Wei
    • 1
  • Yuan Wang
    • 1
    • 2
    • 3
  • Huaqing Zhu
    • 1
    • 2
    • 3
  1. 1.Institute of Clinical PharmacologyAnhui Medical UniversityHefeiPeople’s Republic of China
  2. 2.Laboratory of Molecular Biology and Department of BiochemistryAnhui Medical UniversityHefeiPeople’s Republic of China
  3. 3.Key Laboratory of Gene Research of Anhui ProvinceHefeiPeople’s Republic of China

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