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Molecular and Cellular Biochemistry

, Volume 398, Issue 1–2, pp 11–19 | Cite as

Knockdown of ANLN by lentivirus inhibits cell growth and migration in human breast cancer

  • Weibing Zhou
  • Zhan Wang
  • Ni Shen
  • Weiwei Pi
  • Wuzhong Jiang
  • Juan Huang
  • Yuanping Hu
  • Xiong Li
  • Lunquan SunEmail author
Article

Abstract

Anillin (ANLN), an actin-binding protein, is required for cytokinesis. Recently, ANLN has been identified as a biomarker in diverse human cancers; however, the precise role of ANLN in breast cancer remains unclear. In this study, we firstly detected the expression of ANLN in 71 patients with breast cancer by immunohistochemistry, and found ANLN was highly expressed in breast cancer tissues. To evaluate the function of ANLN in breast cancer cells, we employed lentivirus-mediated RNA interference to knock down ANLN expression in two human breast cancer cell lines, MDA-MB-231, and ZR-75-30. Knockdown of ANLN remarkably inhibited the proliferation rate and colony formation ability of both breast cancer cell lines. Moreover, flow cytometry analysis showed that depletion of ANLN in MDA-MB-231 cells blocked the cell cycle progression, with more cells delayed at G2/M phase, due to phosphorylation of Cdc2 and suppression of Cyclin D1. Furthermore, knockdown of ANLN strongly suppressed the migration of breast cancer cells, strengthening the evidence that ANLN could be involved in breast cancer progression. Our results may suggest ANLN as a potential target candidate in breast cancer.

Keywords

Anillin Breast cancer Immunohistochemistry RNA interference Proliferation 

Notes

Acknowledgments

This study was supported by a Grant from the National Natural Science Foundation of China (81372842).

Supplementary material

11010_2014_2200_MOESM1_ESM.docx (11 kb)
Supplementary material 1 (DOCX 10 kb)
11010_2014_2200_MOESM2_ESM.tif (552 kb)
Supplementary material 2 (TIFF 552 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Weibing Zhou
    • 1
  • Zhan Wang
    • 1
  • Ni Shen
    • 1
  • Weiwei Pi
    • 1
  • Wuzhong Jiang
    • 1
  • Juan Huang
    • 2
  • Yuanping Hu
    • 2
  • Xiong Li
    • 3
  • Lunquan Sun
    • 3
    Email author
  1. 1.Department of Oncology, Xiangya HospitalCentral South UniversityChangshaChina
  2. 2.Hunan Province Clinic Meditech Research Center for Breast Cancer, Xiangya HospitalCentral South UniversityChangshaChina
  3. 3.Center for Molecular Medicine, Xiangya HospitalCentral South UniversityChangshaChina

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