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Molecular and Cellular Biochemistry

, Volume 396, Issue 1–2, pp 117–128 | Cite as

MicroRNA-204, a direct negative regulator of ezrin gene expression, inhibits glioma cell migration and invasion

  • Jie Mao
  • Mengying Zhang
  • Min Zhong
  • Yingying Zhang
  • Kun LvEmail author
Article

Abstract

Ezrin is overexpressed in a variety of neoplastic cells and involved in the later stages of tumor progression and metastasis. Ezrin expression can be regulated at both the transcriptional and post-transcriptional levels. We used a combination of bioinformatics and experimental techniques to demonstrate that the miR-204 is a direct negative regulator of ezrin. Overexpression of miR-204 mimics decreased the activity of a luciferase reporter containing the ezrin 3′ UTR and led to repression of ezrin protein. In contrast, ectopic expression of miR-204 inhibitor elevated ezrin expression. We also show that miR-204 is down-regulated in a panel of glioma tissues and in high invasive glioma cell lines we examined. Moreover, miR-204 mimics significantly reduced glioma cell migration and invasion, while miR-204 inhibitor generated the opposite results. Finally, overexpression of miR-204 and knockdown of ezrin reduced glioma cell invasion, and these effects could be rescued by re-expression of ezrin. These findings reveal that miR-204 could be partly due to its inhibitory effects on glioma cell migration and invasion through regulating ezrin expression.

Keywords

MiR-204 Glioma Ezrin Migration Invasion 

Notes

Acknowledgments

This work was supported by National Natural Science Foundation of China (81300172 to K.L., 81301497 to Y.Y.Z.); Natural Science Foundation of Anhui Province (1308085QH137 to K.L., 1408085MH205 to J.M., 1408085QH148 to Y.Y.Z.).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Jie Mao
    • 1
  • Mengying Zhang
    • 2
  • Min Zhong
    • 2
  • Yingying Zhang
    • 3
  • Kun Lv
    • 2
    Email author
  1. 1.Department of Neurosurgery of The first affiliated HospitalWannan Medical CollegeWuhuPeople’s Republic of China
  2. 2.Central Laboratory of The first affiliated HospitalWannan Medical CollegeWuhuPeople’s Republic of China
  3. 3.Laboratory Medicine of The first affiliated HospitalWannan Medical CollegeWuhuPeople’s Republic of China

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