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Knockdown of NOB1 expression inhibits the malignant transformation of human prostate cancer cells

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Abstract

Nin one binding-1 protein (NOB1) is a kind of zinc protein involved in ribosome biogenesis and controlled proteolysis. To explore the function of NOB1 in human prostate malignancy, we analyzed the expression of NOB1 in prostate cancer and found that NOB1 was elevated in prostate cancer tissues compared to the adjacent normal tissues. Knockdown of NOB1 by lentivirus-shRNA inhibited the proliferation and colony-formation ability of PC-3 and DU145 prostate cancer cells. Cell cycle analysis showed that silencing of NOB1 caused G0/G1 phase arrest and a slight decrease in S phase (P < 0.05). Furthermore, knockdown of NOB1 significantly suppressed the mobility of PC-3 and DU145 prostate cancer cells (P < 0.05). Collectively, these findings suggested that NOB1 might be involved in tumorigenecity of prostate cancer, and could be a potential molecular target for prostate cancer gene therapy.

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (Nos. 30973006, 81170637), National Natural Science Foundation of China for Youths (Nos. 81001136 and 81202020), Shanghai Committee of Science and Technology General Program for Medicine (No. 11JC1402302), Key Project of Science and Innovation Foundation of Shanghai Ministry of Education (14zz084), and Military Fund for Health Care (13BJZ29).

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Correspondence to Danfeng Xu or Xingang Cui.

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Supplementary Fig. 1

Tumorigenesis in nude mice. DU145 cells were transducted with Lv-shcon or Lv-shNOB1. Cell suspensions (200 µL) were subcutaneously injected at a density of 5 × 107/mL into nude mice, and the tumor volumes were monitored at the indicated intervals (A). Tumor volume was calculated with the formula V = (a 2 × b)/2 where a is the width of the tumor (small diameter), and b the length (large diameter), both in millimeters. At day 26, the mice were sacrificed and the tumors were isolated (C). Tumor weights were detected (B). (JPEG 46 kb)

Supplementary Fig. 2

NOB1 knockdown did not significantly influence the apoptosis of prostate cancer cells. (JPEG 43 kb)

Supplementary Fig. 3

The original data of flow cytometry analysis of cell cycle distribution after NOB1 knockdown. (JPEG 138 kb)

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Zhang, X., Zhang, D., Qu, F. et al. Knockdown of NOB1 expression inhibits the malignant transformation of human prostate cancer cells. Mol Cell Biochem 396, 1–8 (2014). https://doi.org/10.1007/s11010-014-2126-z

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  • DOI: https://doi.org/10.1007/s11010-014-2126-z

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