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Molecular and Cellular Biochemistry

, Volume 386, Issue 1–2, pp 153–165 | Cite as

Isoorientin attenuates lipopolysaccharide-induced pro-inflammatory responses through down-regulation of ROS-related MAPK/NF-κB signaling pathway in BV-2 microglia

  • Li Yuan
  • Yuchen Wu
  • Xiaomeng Ren
  • Qian Liu
  • Jing Wang
  • Xuebo LiuEmail author
Article

Abstract

Isoorientin (ISO) is a flavonoid compound in the human diet, and has been known to possess various bioactivities. However, the effects of ISO on microglia inflammation have not been investigated. The current study investigates the neuroprotective effect of ISO in LPS-activated mouse microglial (BV-2) cells. ISO significantly increased the BV-2 cells viability, blocked the protein expression of inducible nitric oxide synthase and cyclooxygenase-2, and decreased the production of nitric oxide, pro-inflammatory cytokines including tumor necrosis factor-α and interleukin-1β. The activation of mitogen-activated protein kinases (MAPKs) was blocked by ISO, and NF-κB nuclear translocation was decreased by ISO both alone and together with NF-κB inhibitor (PDTC) and MAPKs inhibitors (U0126, SP 600125, and SB 203580). Furthermore, ISO strongly quenched intracellular reactive oxygen species (ROS) generation. ROS inhibitor (N-acetyl cysteine, NAC) significantly inhibited pro-inflammatory cytokines release and NF-κB and MAPKs activation, indicating that ISO attenuated neuroinflammation by inhibiting the ROS-related MAPK/NF-κB signaling pathway.

Keywords

Isoorientin Neuroinflammatory Microglia MAPK kinases NF-κB ROS 

Abbreviations

BV-2 cell

Mouse microglial cell line

ISO

Isoorientin

LPS

Lipopolysaccharide

ROS

Reactive oxygen species

NO

Nitric oxide

MAPKs

Mitogen-activated protein kinases

NF-κB

Nuclear factor κB

TNF-α

Tumor necrosis factor

IL

Interleukin

iNOS

Inducible nitric oxide synthase

COX

Cyclooxygenase

Notes

Acknowledgments

This work was financially supported by the Young Scientists Fund of the National Natural Science Foundation of China (No. 31000757), and the National “Twelfth Five-Year” Plan for Science and Technology Support (No. 2012BAH30F03).

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

11010_2013_1854_MOESM1_ESM.ppt (450 kb)
Supplementary material 1 (PPT 450 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Li Yuan
    • 1
  • Yuchen Wu
    • 1
  • Xiaomeng Ren
    • 1
  • Qian Liu
    • 1
  • Jing Wang
    • 1
  • Xuebo Liu
    • 1
    Email author
  1. 1.Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and EngineeringNorthwest A&F UniversityYanglingChina

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