Molecular and Cellular Biochemistry

, Volume 380, Issue 1–2, pp 249–257 | Cite as

The activation of HMGB1 as a progression factor on inflammation response in normal human bronchial epithelial cells through RAGE/JNK/NF-κB pathway

  • Xiaojin Wu
  • Yanyan Mi
  • Hui Yang
  • Ankang Hu
  • Qingguo Zhang
  • Chunli Shang
Article

Abstract

Extracellular high-mobility group box-1 (HMGB-1) has been implicated in the inflammation response leading to the precancerous lesions of non-small cell lung cancer (NSCLC). However, the role of HMGB-1 in the inflammation response in normal human bronchial epithelial (NHBE) cells and its underlying mechanisms were still not fully understood. In this study, the inflammation response in NHBE cells was stimulated by 2.5, 5, and 10 μg/ml HMGB-1. However, the receptor for advanced glycation end products (RAGE) blocker RAGE-Ab (5 μg/ml) or 10 μM c-Jun N-terminal kinases (JNK) inhibitor SP600125 could inhibit HMGB1-induced the release of inflammation cytokines including TNF-α, IL-8, IL-10, and MCP-1 in a dose-dependent manner. Furthermore, HMGB1-induced RAGE protein expression, JNK and NF-κB activation were attenuated by the pretreatment with RAGE-Ab or JNK inhibitor SP600125 in Western blot analysis. Our data indicated that HMGB-1 induced inflammation response in NHBE cells through activating RAGE/JNK/NF-κB pathway. HMGB-1 could act as a therapeutic target for inflammation leading NHBE cells to the precancerous lesions of NSCLC.

Keywords

HMGB-1 Inflammation Normal human bronchial epithelial cells NSCLC RAGE JNK 

Notes

Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No. 81172503).

Conflict of interest

We declare that there is no conflict of interest. No author has any financial interest or conflict of interest involved with this study.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Xiaojin Wu
    • 1
  • Yanyan Mi
    • 2
  • Hui Yang
    • 3
  • Ankang Hu
    • 2
  • Qingguo Zhang
    • 3
  • Chunli Shang
    • 4
  1. 1.Department of Radiation OncologyThe First People’s Hospital of XuzhouXuzhouPeople’s Republic of China
  2. 2.Department of PharmacyXuzhou Medical CollegeXuzhouPeople’s Republic of China
  3. 3.Department of NeurosurgeryThe First People’s Hospital of XuzhouXuzhouPeople’s Republic of China
  4. 4.Department of RespiratoryThe First People’s Hospital of XuzhouXuzhouPeople’s Republic of China

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