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Molecular and Cellular Biochemistry

, Volume 374, Issue 1–2, pp 37–48 | Cite as

Catechin induced modulation in the activities of thyroid hormone synthesizing enzymes leading to hypothyroidism

  • Amar K. ChandraEmail author
  • Neela De
Article

Abstract

Catechins, the flavonoids found in abundance in green tea, have many beneficial health effects such as antioxidative, anticarcinogenic, anti-inflammatory, antiallergic, and hypotensive properties. However, flavonoids have antithyroid/goitrogenic effect, although less information is available about the effect of pure catechin on thyroid physiology. The present investigation has been undertaken to explore the effect of catechin administration on thyroid physiology in rat model. For the in vivo experiment catechin was injected intraperitoneally (i.p.) at doses of 10, 20 and 30 mg/kg body to male albino rats for 15 and 30 days, respectively, and thyroid activities were evaluated with respect to determination of serum levels of thyroid hormones, thyroid peroxidase, 5′-deiodinase I (5′-DI), and Na+, K+-ATPase activities that are involved in the synthesis of thyroid hormone. Catechin decreased the activities of thyroid peroxidase and thyroidal 5′-deiodinase I, while Na+, K+-ATPase activity significantly increased in dose-dependent manner; substantial decrease in serum T3 and T4 levels coupled with significant elevation of serum TSH were also noted. Histological examinations of the thyroid gland revealed marked hypertrophy and/or hyperplasia of the thyroid follicles with depleted colloid content. In in vitro study, short-term exposure of rat thyroid tissue to catechin at the concentrations of 0.10, 0.20, and 0.30 mg/ml leads to decrease in the activities of thyroid peroxidase and 5′-deiodinase I, while the activity of thyroidal Na+, K+-ATPase remains unaltered even at high concentration of catechin treatment. The present study reinforces the concept that catechin, tea flavonoids possess potent antithyroid activity as evidenced from in vivo and in vitro studies.

Keywords

Catechins Thyroid hormones Thyroid peroxidase Na+, K+-ATPase 5′-Deiodinase I 

Abbreviations

ANOVA

Analysis of variance

BSA

Bovine serum albumin

BTE

Black tea extract

COMT

Catechol-O-methyltransferase

DIT

Diiodotyrosine

DMSO

Dimethylsulfoxide

DTE

Dithioerythritol

EC

(−)-Epicatechin

ECG

(−)-Epicatechin gallate

EDTA

Ethylenediamine tetraacetic acid

EGC

(−)Epigallocatechin

EGCG

(−)-Epigallocatechin gallate

ELISA

Enzyme-linked immuno sorbent assay

GTC

Green tea catechins

GTE

Green tea extract

GTP

Green tea polyphenols

H&E

Hematoxylin & Eosin

H2O2

Hydrogen peroxide

MIT

Monoiodotyrosine

MTT

3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltrazolium bromide

Na+, K+-ATPase

Sodium, Potassium Adenosine Triphosphatase

Na2ATP

Adenosine-5′-triphosphoräure Dinatriumsalz

NIDDK

National Institute of Diabetes and Digestive and Kidney Diseases

NIS

Sodium-Iodide Symporter

PTU

Propylthiouracil

RIA

Radioimmunoassay

rT3

Reverse T3

SDS

Sodium dodecyl sulfate

T3

Tri-iodothyronine

T4

Thyroxin

TBG

Thyroxine-binding globulin

TPO

Thyroid peroxidase

TRH

Thyrotropin-releasing Hormone

Tris-ATP

Hydroxymethyl Aminomethane Hydrochloride Adenosine Triphosphate

TSH

Thyroid stimulating hormone

5′-DI

5′-Monodeiodinase-I

Notes

Acknowledgments

Acknowledgement is due to the National Tea Research Foundation (NTRF), Kolkata, India, for providing fellowship to Neela De for this work. The authors are grateful to Prof. Arun K. Ray (Bose Institute, India) for his contribution in conducting enzyme study. The authors thank Dr Syed N Kabir, Scientist, Cell Biology & Physiology Division, Indian Institute of Chemical Biology (IICB), Kolkata, India, for his help in conducting RIA of TSH.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.Endocrinology & Reproductive Physiology Laboratory, Department of PhysiologyUniversity of CalcuttaKolkataIndia
  2. 2.University College of Science & TechnologyKolkataIndia

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