Molecular and Cellular Biochemistry

, Volume 374, Issue 1–2, pp 13–20 | Cite as

Wnt/β-catenin signaling induces the aging of mesenchymal stem cells through promoting the ROS production

  • Da-yong Zhang
  • Yu Pan
  • Chong Zhang
  • Bing-xi Yan
  • Shan-shan Yu
  • Dong-ling Wu
  • Meng-meng Shi
  • Kai Shi
  • Xin-xiao Cai
  • Shuang-shuang Zhou
  • Jun-bo WangEmail author
  • Jian-ping Pan
  • Li-huang Zhang


Recent studies have demonstrated that the Wnt/β-catenin signaling plays an important role in stem cell aging. However, the mechanisms of cell senescence induced by Wnt/β-catenin signaling are still poorly understood. Our preliminary study has indicated that activated Wnt/β-catenin signaling can induce MSC aging. In this study, we reported that the Wnt/β-catenin signaling was a potent activator of reactive oxygen species (ROS) generation in MSCs. After scavenging ROS with N-acetylcysteine, Wnt/β-catenin signaling-induced MSC aging was significantly attenuated and the DNA damage and the expression of p16INK4A, p53, and p21 were reduced in MSCs. These results indicated that the Wnt/β-catenin signaling could induce MSC aging through promoting the intracellular production of ROS, and ROS may be the main mediators of MSC aging induced by excessive activation of Wnt/β-catenin signaling.


Mesenchymal stem cells (MSCs) Aging Wnt/β-catenin signaling ROS 



Mesenchymal stem cells






Superoxide dismutase




Sodium dodecyl sulfate–polyacrylamide gel electrophoresis


Fluorescein isothiocyanate





The authors gratefully acknowledge financial support from the Teachers Research Fund of Zhejiang University City College (J-12019, J-12021), the Student Research Fund of Zhejiang University City College (XZ2012562091, X2012562098), the Zhejiang Provincial Foundation of National Science (LQ12H31001), and the Science Research Foundation of the Zhejiang Health Bureau (2012KYA068, 2012KYB066).

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

11010_2012_1498_MOESM1_ESM.doc (594 kb)
Supplementary material 1 (DOC 593 kb)


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Da-yong Zhang
    • 1
  • Yu Pan
    • 1
  • Chong Zhang
    • 1
  • Bing-xi Yan
    • 1
  • Shan-shan Yu
    • 1
  • Dong-ling Wu
    • 1
  • Meng-meng Shi
    • 1
  • Kai Shi
    • 1
  • Xin-xiao Cai
    • 1
  • Shuang-shuang Zhou
    • 1
  • Jun-bo Wang
    • 1
    Email author
  • Jian-ping Pan
    • 1
  • Li-huang Zhang
    • 1
  1. 1.Department of Basic MedicineSchool of Medicine and Life Sciences, Zhejiang University City CollegeHangzhouPeople’s Republic of China

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