Protective effect of gallic acid isolated from Peltiphyllum peltatum against sodium fluoride-induced oxidative stress in rat’s kidney
- 695 Downloads
In the present study, the nephroprotective effect of gallic acid isolated from Peltiphyllum peltatum was examined in sodium fluoride (NaF) treated rats. Nephrotoxicity was induced by 1-week intoxication of NaF at 600 ppm through drinking water. The levels of thiobarbituric acid reactive substances, reduced glutathione as well as activities of superoxide dismutase and catalase in renal tissues homogenates were determined. The serum biochemical markers of renal injuries including creatinine, serum urea, blood urea nitrogen, uric acid levels as well as the levels of phosphate and calcium were also assessed. Intoxication with NaF caused a significant increase in the levels of thiobarbituric acid reactive substances (46 % versus to control) and reduced the glutathione concentration (47 %) and the activities of superoxide dismutase (46 %) and catalase (41 %) in renal tissues homogenates. NaF intoxication also induced significant alterations in the kidney biochemical markers increasing the levels of urea, uric acid, blood urea nitrogen, creatinine, and phosphate and decreasing the levels of calcium. Daily administration of gallic acid (20 mg/kg) for 1 week before NaF intoxication brought the antioxidant–oxidant balance similar to the NaF-untreated group. Silymarin, used a standard antioxidant agent, also showed a nephroprotective activity. We concluded that NaF caused nephrotoxicity and oxidative stress in renal tissues and daily administration of gallic acid for 1 week prior to intoxication inhibited toxicity and oxidative stress.
KeywordsGallic acid Oxidative stress Sodium fluoride Nephrotoxicity
This study was partly supported by the research grant of University of Greenwich (UK) and National Elite’s Foundation of Iran (Iran) for this study. We also dedicate this paper to Mrs. Seyed Maryam Nabavi and to memory of Mr. Seyed Ali Asghar Nabavi and victims of Azerbaijan earthquake (August 11, 2012; Iran).
- 10.Habtemariam S (2008) Activity-guided isolation and identification of antioxidant components from ethanolic extract of Peltiphyllum peltatum (Torr.) Engl. Nat Prod Commun 3:1321–1324Google Scholar
- 21.Inkielewicz I, Krechniak J (2003) Fluoride content in soft tissues and urine of rats exposed to sodium fluoride in drinking water. Fluoride 36:263–266Google Scholar
- 22.Shivarajashankara YM, Shivashankara AR, Bhat PG, Rao PG (2001) Effect of fluoride intoxication on lipid peroxidation and antioxidant systems in rats. Fluoride 34:108–113Google Scholar
- 26.Samanidou V, Tsagiannidis A, Sarakatsianos I (2012) Simultaneous determination of polyphenols and major purine alkaloids in Greek Sideritis species, herbal extracts, green tea, black tea, and coffee by high-performance liquid chromatography-diode array detection. J Sep Sci 35:608–615PubMedCrossRefGoogle Scholar
- 30.Stenlake JB (1979) Foundations of molecular pharmacology, vol 1: medicinal and pharmaceutical chemistry, vol 1. The Athlone Press of the University of London, LondonGoogle Scholar
- 31.Andersson CM, Hallberg A, Hogberg T (1996) Advances in the development of pharmaceutical antioxidants. In: Testa B, Meyer VA (eds) Advances in drug research. Academic Press, London, pp 65–180Google Scholar