Molecular and Cellular Biochemistry

, Volume 372, Issue 1–2, pp 47–55 | Cite as

Changes in plasma thiol levels induced by different phases of treatment in breast cancer; the role of commercial extract from black chokeberry

  • Magdalena Kędzierska
  • Rafał Głowacki
  • Urszula Czernek
  • Katarzyna Szydłowska-Pazera
  • Piotr Potemski
  • Janusz Piekarski
  • Arkadiusz Jeziorski
  • Beata OlasEmail author


Different low-molecular-weight thiols, including glutathione, cysteine, and cysteinylglycine are physiological free radical scavengers. On the other hand, homocysteine may play a role as an oxidant. The aim of our present study was to establish in vitro the effects of the commercial extract of Aronia melanocarpa (Aronox®) on the amount of selected low-molecular-weight thiols and the activity of antioxidative enzymes (superoxide dismutase, glutathione peroxidase, and glutathione reductase) in plasma obtained from patients with invasive breast cancer during different phases of treatment [before or after the surgery and patients after different phases of chemotherapy (doxorubicin and cyclophosphamide)] and from healthy subjects. Patients were hospitalized in Department of Oncological Surgery and Department of Chemotherapy, Medical University of Lodz, Poland. The level of low-molecular-weight thiols was determined by high-performance liquid chromatography. We observed that in the presence of the Aronia extract changes in amount of thiols in plasma from breast cancer patients (at all tested groups) were significantly reduced. Our results showed that tested commercial extract reduced modifications of antioxidative enzymes activity in plasma from patients during different phases of treatment, but this effect was not statistical significant. Our results suggest that the Aronia extract supplementation in breast cancer patients has a beneficial effect on thiols concentration in plasma. Plasma, as reported in this work, could be used as an experimental model to evaluate the beneficial action of plant supplements, including phenolic extracts on thiols or other molecules during different phases of treatment.


Breast cancer Plasma Chemotherapy Thiols Aronia 



Supported by the Grant 506/810 from University of Lodz, Poland.

Conflict of interest

None to declare.


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Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Magdalena Kędzierska
    • 1
    • 3
  • Rafał Głowacki
    • 2
  • Urszula Czernek
    • 3
  • Katarzyna Szydłowska-Pazera
    • 3
  • Piotr Potemski
    • 3
  • Janusz Piekarski
    • 4
  • Arkadiusz Jeziorski
    • 4
  • Beata Olas
    • 1
    Email author
  1. 1.The Department of General Biochemistry, Faculty of Biology and Environmental ProtectionUniversity of LodzLodzPoland
  2. 2.The Department of Environmental ChemistryUniversity of LodzLodzPoland
  3. 3.The Department of ChemotherapyMedical University of Lodz, Copernicus Memorial HospitalLodzPoland
  4. 4.The Department of Surgical OncologyMedical University of Lodz, Copernicus Memorial HospitalLodzPoland

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