Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of ∙NO
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In this study, the effects of l-arginine-nitric-oxide (∙NO)-producing pathway on protein content of ubiquitin, as an important component of ubiquitin–proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide (∙NO), besides well established roles, is involved in this aspect of structure remodeling, as well.
Keywordsl-Arginine Nitric oxide Ubiquitin Brown adipose tissue Cold-acclimation
This study was supported by the Ministry of Education and Science of the Republic of Serbia, Grant No. 173055. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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