Molecular and Cellular Biochemistry

, Volume 364, Issue 1–2, pp 181–191 | Cite as

Excessive dietary calcium in the disruption of structural and functional status of adult male reproductive system in rat with possible mechanism

  • Amar K Chandra
  • Pallav Sengupta
  • Haimanti Goswami
  • Mahitosh Sarkar


Calcium is essential for functioning of different systems including male reproduction. However, it has also been reported as chemo-castrative agent. The study has been undertaken to elucidate the effect of excessive dietary calcium on male reproductive system in animals with possible action. Adult male healthy rats fed CaCl2 at different doses (0.5, 1.0 and 1.5 g%) in diet for 13 and 26 days to investigate reproductive parameters as well as the markers of oxidative stress. Significant alteration was found (P < 0.05) in testicular and accessory sex organs weight, epididymal sperm count, testicular steroidogenic enzyme (Δ5 3β-HSD and 17β-HSD) activities, serum testosterone, LH, FSH, LPO, activities of antioxidant enzymes, testicular histoarchitecture along with adrenal Δ5 3β-HSD activity with corticosterone level in dose- and time-dependent manner. Overall observations suggest that excessive dietary calcium enhances the generation of free-radicals resulting in structural and functional disruption of male reproduction.


Calcium chloride Steroidogenic enzyme Oxidative stress Antioxidant enzyme Lipid peroxidation Superoxide dismutase 



Reactive oxygen species


Type A spermatogonia


Preleptotene spermatocytes


Mid-pachytene spermatocytes


Step 7 spermatids


Luteinizing hormone


Follicle stimulating hormone


Hydroxysteroid dehydrogenase


Nicotinamide adenine dinucleotide phosphate


Lipid peroxidation


Thiobarbituric acid reactive substances




Superoxide dismutase




Ethylenediamine tetraacetic acid


Bovine serum albumin




Analysis of variance


Hypothalamo–pituitary–gonadal axis


Hypothalamo–pituitary–adrenal axis


Paraventricular nucleus


Corticotrophin releasing hormone


Adreno-corticotropic hormone


Poly unsaturated fatty acid


Inositol tri-phosphate



Acknowledgment is due to the Project of University Potential for Excellence (UPE), University Grants Commission (UGC), New Delhi, India for providing scholarship to second author for conducting this work. The authors are thankful to Dr. Syed N. Kabir, Scientist, Cell Biology & Physiology Division, Indian Institute of Chemical Biology (IICB), Kolkata, India for his help in conducting RIA of FSH and LH.

Conflict of interest



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Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Amar K Chandra
    • 1
  • Pallav Sengupta
    • 1
  • Haimanti Goswami
    • 1
  • Mahitosh Sarkar
    • 2
  1. 1.Endocrinology & Reproductive Physiology Laboratory, Department of PhysiologyUniversity College of Science & Technology, University of CalcuttaKolkataIndia
  2. 2.Department of PhysiologyGurunanak Institute of Dental Science & ResearchKolkataIndia

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