Molecular and Cellular Biochemistry

, Volume 363, Issue 1–2, pp 437–444

Function of the syndecan-4 cytoplasmic domain in oligomerization and association with α-actinin in turkey muscle satellite cells

  • Jonghyun Shin
  • Yan Song
  • Douglas C. McFarland
  • Sandra G. Velleman
Article

DOI: 10.1007/s11010-011-1198-2

Cite this article as:
Shin, J., Song, Y., McFarland, D.C. et al. Mol Cell Biochem (2012) 363: 437. doi:10.1007/s11010-011-1198-2

Abstract

Syndecan-4 (S4) is a cell membrane heparan sulfate proteoglycan that plays a role in satellite cell mediated myogenesis. S4 modulates the proliferation of myogenic satellite cells, but the mechanism of how S4 functions during myogenesis is not well understood. In other cell systems, S4 has been shown to form oligomers in the cell membrane and interact through its cytoplasmic domain with the cytoskeletal protein α-actinin. This study addressed if S4 forms oligomers and interacts with α-actinin in muscle. The S4 cytoplasmic domain was found to interact with α-actinin in a phosphatidylinositol-4,5-bisphosphate dependent manner, but did not associate with vinculin. Through confocal microscopy, both S4 and syndecan-4 without the cytoplasmic domain were localized to the cell membrane. Although the cytoplasmic domain was necessary for the interaction with α-actinin, S4 oligomer formation occurred in the absence of the cytoplasmic domain. These data indicated that S4 function in skeletal muscle is mediated through the formation of oligomers and interaction with the cytoskeletal protein α-actinin.

Keywords

α-Actinin Muscle Oligomers Satellite cell Syndecan-4 

Abbreviations

BSA

Bovine serum albumin

FGF2

Fibroblast growth factor 2

IPTG

Isopropyl-β-1-thiogalactopyranoside

IP

Immunoprecipitated

PKCα

Protein kinase C α

PVDF

Polyvinylidene fluoride membrane

PIP2

Phosphatidylinositol-4,5-bisphosphate

siRNA

Small interfering RNA

SDS-PAGE

Sodium dodecyl sulfate-polyacrylamide gel

S4

Syndecan-4

S4C

Syndecan-4 without the cytoplasmic domain

TBS-T

Tris-buffered saline containing Tween 20

Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Jonghyun Shin
    • 1
  • Yan Song
    • 1
  • Douglas C. McFarland
    • 2
  • Sandra G. Velleman
    • 1
  1. 1.Department of Animal Sciences, Ohio Agricultural Research and Development CenterThe Ohio State UniversityWoosterUSA
  2. 2.Department of Animal ScienceSouth Dakota State UniversityBrookingsUSA

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