Molecular and Cellular Biochemistry

, Volume 363, Issue 1, pp 245–255

External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells

  • Xin Yan
  • Feng Li
  • Igor Dozmorov
  • Mark Barton Frank
  • Ming Dao
  • Michael Centola
  • Wei Cao
  • Dan Hu
Article

DOI: 10.1007/s11010-011-1176-8

Cite this article as:
Yan, X., Li, F., Dozmorov, I. et al. Mol Cell Biochem (2012) 363: 245. doi:10.1007/s11010-011-1176-8

Abstract

Small-cell lung cancer (SCLC) is a highly malignant carcinoma with poor long-term survival. Effective treatment remains highly demanded. In the present study, we demonstrated that External Qi of Yan Xin Qigong (YXQ-EQ) exerted potent cytotoxic effect towards SCLC cell line NCI-H82 via induction of apoptosis. Global gene expression profiling identified 39 genes whose expression was altered by YXQ-EQ in NCI-82 cells. Among them, semi-quantitative RT-PCR and real-time qPCR analyses confirmed that the gene expression levels of apoptotic proteins death-associated protein kinase 2 and cell death-inducing DFFA-like effector b were upregulated, whereas that of oncoproteins DEK and MYCL1, cell migration-promoting proteins CD24 and integrin-alpha 9, and glycolytic enzyme aldolase A were downregulated. These findings suggest that YXQ-EQ may exert anticancer effect through modulating gene expression in a way that facilitates cancer cell apoptosis while represses proliferation, metastasis, and glucose metabolism.

Keywords

Small-cell lung cancer Gene expression Cell death Anti-cancer effect 

Abbreviations

SCLC

Small-cell lung cancer

TCM

Traditional Chinese medicine

YXQ

Yan Xin Qigong

YXQ-EQ

External Qi of Yan Xin Qigong

FITC

Fluorescein isothiocyanate

PI

Propidium iodide

ITGA9

Integrin alpha 9

VCAM1

Vascular-cell-adhesion molecule 1

GRKs

G-protein-coupled receptor kinases

MYCL1 (or L-Myc)

Lung myelocytomatosis viral oncogene homolog 1

DAPK2

Death-associated protein kinase 2

CIDE-B

Cell death-inducing DFFA-like effector b

CAR

Cancer-associated retinopathy

RCVRN

Recoverin

ALDOA

Aldolase A

Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Xin Yan
    • 1
    • 2
  • Feng Li
    • 3
  • Igor Dozmorov
    • 4
  • Mark Barton Frank
    • 4
  • Ming Dao
    • 5
  • Michael Centola
    • 4
  • Wei Cao
    • 3
  • Dan Hu
    • 6
  1. 1.The Institute of Chongqing Traditional Chinese MedicineChongqingChina
  2. 2.New Medical Science Research InstituteNew YorkUSA
  3. 3.University of Oklahoma Health Sciences CenterOklahomaUSA
  4. 4.Microarray Research FacilityOklahoma Medical Research FoundationOklahomaUSA
  5. 5.Department of Materials Science and EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  6. 6.Center for Neurologic DiseasesBrigham and Women’s Hospital, Harvard Medical SchoolBostonUSA

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