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RETRACTED ARTICLE: Targeting of colorectal cancer growth, metastasis, and anti-apoptosis in BALB/c nude mice via APRIL siRNA

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This article was retracted on 17 October 2022

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Abstract

A proliferation-inducing ligand (APRIL) is overexpressed in most tumor cells and tissues, especially in tumors of the alimentary system, such as colorectal cancer (CRC), gastric cancer, and liver cancer. RNA interference (RNAi) has been proved to be a powerful tool for gene knockdown and holds great promise for the treatment of cancer. In this study, the efficacy of RNAi targeting APRIL was analyzed via relevant experiments on human CRC xenografted in BALB/c nude mice. Both the mRNA and protein levels of APRIL were examined after intratumoral injection of APRIL small interfering RNA (siRNA). Meanwhile, pathological tools were utilized to observe the alterations on the aspects of proliferation, metastasis, apoptosis and cellular necrosis by means of detecting proliferating cell nuclear antigen, Ki-67, MMP-2, MMP-9, TIMP-3, TIMP-4, Bcl-2, Bax and Bcl-xL of CRC. In addition, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) and hematoxylin and eosin staining were also conducted to examine cell apoptosis and necrosis. It was found that grafted human colorectal tumor growth and metastasis were obviously inhibited while tumor cell apoptosis and necrosis were induced after in vivo APRIL siRNA injection into nude mice. The data indicated that silencing of the APRIL gene using RNAi may serve as a novel therapeutic strategy for treatment of CRC.

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Acknowledgment

This work was supported by the Key Subject of Jiangsu Province (XK20070302).

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Authors and Affiliations

  1. Medical Laboratory Center, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China

    Jingchun Wang, Weifeng Ding, Baolan Sun, Rongrong Jing & Huimin Wang

  2. School of Public Health, Nantong University, Nantong, 226001, Jiangsu Province, China

    Jingchun Wang, Weifeng Ding & Huimin Wang

  3. Pathology Medicine Center, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China

    Hua Huang & Gongsheng Shi

Authors
  1. Jingchun Wang
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  2. Weifeng Ding
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  3. Baolan Sun
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  4. Rongrong Jing
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  5. Hua Huang
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  6. Gongsheng Shi
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  7. Huimin Wang
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Corresponding author

Correspondence to Huimin Wang.

Additional information

Jingchun Wang and Weifeng Ding contributed equally to this work.

Electronic supplementary material

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11010_2011_1151_MOESM1_ESM.tif

Supplementary Figure 1a SW480 cells transfected with shAPRIL had lower growing velocity in nude mice (n = 5). a The tendency of tumor growth in different groups. Firstly, SW480 cells were transfected with shAPRIL (sh637 and sh1750) and the pGC vector. After cells were subcutaneously implanted into nude mice, tumor volume was monitored regularly (TIFF 680 kb)

11010_2011_1151_MOESM2_ESM.tif

Supplementary Figure 1b SW480 cells transfected with shAPRIL had lower growing velocity in nude mice (n = 5). b Tumors images from nude mice (A, sh637 transfected group; B, sh1750 transfected group; C, pGC vector transfected group; D, SW480 cells untransfected group) (TIFF 1721 kb)

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Wang, J., Ding, W., Sun, B. et al. RETRACTED ARTICLE: Targeting of colorectal cancer growth, metastasis, and anti-apoptosis in BALB/c nude mice via APRIL siRNA. Mol Cell Biochem 363, 1–10 (2012). https://doi.org/10.1007/s11010-011-1151-4

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  • DOI: https://doi.org/10.1007/s11010-011-1151-4

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