Regulation of caspase pathways by protein kinase CK2: identification of proteins with overlapping CK2 and caspase consensus motifs

  • Jacob P. Turowec
  • James S. Duncan
  • Greg B. Gloor
  • David W. Litchfield
Article
First Online:
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Accepted:

Abstract

Apoptosis, or programmed cell death, is a vital cellular process often impaired in diseases such as cancer. Aspartic acid-directed proteases known as caspases cleave a broad spectrum of cellular proteins and are central constituents of the apoptotic machinery. Caspases are regulated by a variety of mechanisms including protein phosphorylation. One intriguing mechanism by which protein kinases can modulate caspase pathways is by blocking substrate cleavage through phosphorylation of residues adjacent to caspase cleavage sites. To explore this mechanism in detail, we recently undertook a systematic investigation using a combination of bioinformatics, peptide arrays, and peptide cleavage assays to identify proteins with overlapping protein kinase and caspase recognition motifs (Duncan et al., Sci Signal 4:ra30, 2011). These studies implicated protein kinase CK2 as a global regulator of apoptotic pathways. In this article, we extend the analysis of proteins with overlapping CK2 and caspase consensus motifs to examine the convergence of CK2 with specific caspases and to identify CK2/caspase substrates known to be phosphorylated or cleaved in cells. Given its constitutive activity and elevated expression in cancer, these observations suggest that the ability of CK2 to modulate caspase pathways may contribute to a role in promoting cancer cell survival and raise interesting prospects for therapeutic targeting of CK2.

Keywords

Protein kinase CK2 Caspase Apoptosis Peptide arrays 
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Notes

Acknowledgments

This study has been funded by an operating grant from the Canadian Institute of Health Research (to DWL). JPT has been supported by scholarships from the Ontario Graduate Scholarship and Ontario Graduate Scholarship Science and Technology programs; JSD was supported by the Canadian Institute of Health Research—Canadian Graduate Scholarship and the Canadian Institute of Health Research—UWO Strategic Training Initiative in Cancer Research and Technology Transfer scholarship. The authors thank all the members of the Litchfield laboratory for holding helpful discussions.

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Jacob P. Turowec
    • 1
  • James S. Duncan
    • 1
  • Greg B. Gloor
    • 1
  • David W. Litchfield
    • 1
    • 2
  1. 1.Department of Biochemistry, Schulich School of Medicine & DentistryUniversity of Western OntarioLondonCanada
  2. 2.Department of Oncology, Schulich School of Medicine & DentistryUniversity of Western OntarioLondonCanada

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